Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

Eliza Ploskon; Sara C. Wagner; Andrew D. Ellington; Michael C. Jewett; Rachel O'Reilly; Paula J. Booth

doi:10.1021/bc500473s

Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

Eliza Ploskon, Sara C. Wagner, Andrew D. Ellington, Michael C. Jewett, Rachel O'Reilly, Paula J. Booth

Chemistry

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

DNA–protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein–protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA–protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation.

Original language	English
Pages (from-to)	427-434
Number of pages	8
Journal	Bioconjugate Chemistry
Volume	26
Issue number	3
DOIs	https://doi.org/10.1021/bc500473s
Publication status	Published - 20 Jan 2015

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abstract = "DNA–protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein–protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA–protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation.",

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AU - O'Reilly, Rachel

AU - Booth, Paula J.

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AB - DNA–protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein–protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA–protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation.

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Controlled Assembly of Artificial Protein-Protein Complexes via DNA Duplex Formation

Abstract

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