Abstract
Molecular markers have been used extensively in studying genetic diversity, genetic relationships and germplasm management. However, the understanding of between and within population genetic variation and how it is partitioned on the basis of geographic origin is crucial as this helps to improve sampling efficiency. The objective of this study was therefore to assess the intra-specific diversity in Oryza longistaminata and how the variation is partitioned within and between different geographic locations, using molecular markers. AFLP analysis generated 176 bands that revealed high levels of polymorphism (95.6%) and diversity within and between populations. The mean Nei's genetic diversity for all the 176 loci in the 48 populations was 0.302 and diversity for populations within countries ranged from 0.1161 to 0.2126. Partitioning of between and within population diversity revealed that the mean allelic diversity at each polymorphic locus was HT=0.3445. The within population diversity was (HS=0.1755) and the between population diversity was (DST=0.1688). Results of AMOVA revealed significant differences (p
Original language | English |
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Pages (from-to) | 308-317 |
Number of pages | 10 |
Journal | African Journal of Biotechnology |
Volume | 4 |
Publication status | Published - 1 Jan 2005 |
Keywords
- Oryza longistaminata
- molecular markers
- AFLP
- populations
- genetic diversity