Configuration of human dendritic cell cytoskeleton by Rho GTPases, the WAS protein, and differentiation

S Burns, AJ Thrasher, MP Blundell, Laura Machesky, GE Jones

Research output: Contribution to journalArticle

247 Citations (Scopus)


The cellular mechanisms that configure the cytoskeleton during migration of dendritic cells (DCs) are poorly understood. Immature DCs assemble specialized adhesion structures known as podosomes at their leading edge; these are associated with the localized recruitment of the Wiskott-Aldrich Syndrome protein (WASp) and the actin organizing actin-related protein 2/3 complex. In immature DCs lacking WASp, podosomes are absent, residual dysmorphic lamellipodia and filopodia are nonpolarized, and migration is severely compromised. Microinjection studies indicate that podosome assembly and polarization require concerted action of Cdc42, Rac, and Rho, thereby providing a link between sequential protrusive and adhesive activity. Formation of podosomes is restricted to cells with an immature phenotype, indicating a specific role for these structures during the early migratory phase. (Blood. 2001;98:1142-1149)
Original languageEnglish
Pages (from-to)1142-1149
Number of pages8
Issue number4
Publication statusPublished - 15 Aug 2001


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