Conditionally essential genes for survival during starvation in Enterococcus faecium E745

Vincent de Maat, Sergio Arredondo-Alonso, Rob J L Willems, Willem van Schaik

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


BACKGROUND: The nosocomial pathogen Enterococcus faecium can survive for prolonged periods of time on surfaces in the absence of nutrients. This trait is thought to contribute to the ability of E. faecium to spread among patients in hospitals. There is currently a lack of data on the mechanisms that are responsible for the ability of E. faecium to survive in the absence of nutrients.

RESULTS: We performed a high-throughput transposon mutant library screening (Tn-seq) to identify genes that have a role in long-term survival during incubation in phosphate-buffered saline (PBS) at 20 °C. A total of 24 genes were identified by Tn-seq to contribute to survival in PBS, with functions associated with the general stress response, DNA repair, metabolism, and membrane homeostasis. The gene which was quantitatively most important for survival in PBS was usp (locus tag: EfmE745_02439), which is predicted to encode a 17.4 kDa universal stress protein. After generating a targeted deletion mutant in usp, we were able to confirm that usp significantly contributes to survival in PBS and this defect was restored by in trans complementation. The usp gene is present in 99% of a set of 1644 E. faecium genomes that collectively span the diversity of the species.

CONCLUSIONS: We postulate that usp is a key determinant for the remarkable environmental robustness of E. faecium. Further mechanistic studies into usp and other genes identified in this study may shed further light on the mechanisms by which E. faecium can survive in the absence of nutrients for prolonged periods of time.

Original languageEnglish
Article number568
Pages (from-to)568
JournalBMC Genomics
Issue number1
Publication statusPublished - 18 Aug 2020


  • Enterococcus faecium
  • Functional genomics
  • Starvation
  • Stress response
  • Tn-seq

ASJC Scopus subject areas

  • Biotechnology
  • Genetics


Dive into the research topics of 'Conditionally essential genes for survival during starvation in Enterococcus faecium E745'. Together they form a unique fingerprint.

Cite this