Complete replication of Hepatitis C virus in cell culture

BD Lindenbach; MJ Evans; AJ Syder; B Wolk; TL Tellinghuisen; CC Liu; T Maruyama; RO Hynes; DR Burton; Jane McKeating; CM Rice

doi:10.1126/science.1114016

Complete replication of Hepatitis C virus in cell culture

BD Lindenbach, MJ Evans, AJ Syder, B Wolk, TL Tellinghuisen, CC Liu, T Maruyama, RO Hynes, DR Burton, Jane McKeating, CM Rice

Immunology and Immunotherapy

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Abstract

Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.

Original language	English
Pages (from-to)	623-6
Number of pages	4
Journal	Science
Volume	309
DOIs	https://doi.org/10.1126/science.1114016
Publication status	Published - 22 Jul 2005

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Cite this

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title = "Complete replication of Hepatitis C virus in cell culture",

abstract = "Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.",

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T1 - Complete replication of Hepatitis C virus in cell culture

AU - Lindenbach, BD

AU - Evans, MJ

AU - Syder, AJ

AU - Wolk, B

AU - Tellinghuisen, TL

AU - Liu, CC

AU - Maruyama, T

AU - Hynes, RO

AU - Burton, DR

AU - McKeating, Jane

AU - Rice, CM

PY - 2005/7/22

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N2 - Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.

AB - Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.

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Complete replication of Hepatitis C virus in cell culture

Abstract

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