Complete high-density lipoproteins in nanoparticle corona

K.A. Dawson, T. Cedervall, E. Hellstrand, A. Andersson, T. Drakenberg, S. Linse, I. Lynch, B. Dahlbäck

Research output: Contribution to journalArticlepeer-review

248 Citations (Scopus)


In a biological environment, nanoparticles immediately become covered by an evolving corona of biomolecules, which gives a biological identity to the nanoparticle and determines its biological impact and fate. Previous efforts at describing the corona have concerned only its protein content. Here, for the first time, we show, using size exclusion chromatography, NMR, and pull-down experiments, that copolymer nanoparticles bind cholesterol, triglycerides and phospholipids from human plasma, and that the binding reaches saturation. The lipid and protein binding patterns correspond closely with the composition of high-density lipoprotein (HDL). By using fractionated lipoproteins, we show that HDL binds to copolymer nanoparticles with much higher specificity than other lipoproteins, probably mediated by apolipoprotein A-I. Together with the previously identified protein binding patterns in the corona, our results imply that copolymer nanoparticles bind complete HDL complexes, and may be recognized by living systems as HDL complexes, opening up these transport pathways to nanoparticles. Apolipoproteins have been identified as binding to many other nanoparticles, suggesting that lipid and lipoprotein binding is a general feature of nanoparticles under physiological conditions.
Original languageEnglish
Pages (from-to)3372-3381
Number of pages10
JournalFEBS Journal
Issue number12
Publication statusPublished - 1 Jun 2009

Bibliographical note

MEDLINE® is the source for the MeSH terms of this document.


Dive into the research topics of 'Complete high-density lipoproteins in nanoparticle corona'. Together they form a unique fingerprint.

Cite this