TY - JOUR
T1 - Comparison of promoter activities in Escherichia coli and Pseudomonas aeruginosa
T2 - use of a new broad-host-range promoter-probe plasmid
AU - Lodge, J
AU - Williams, R
AU - Bell, A
AU - Chan, B
AU - Busby, S
PY - 1990
Y1 - 1990
N2 - The broad-host-range plasmid, pRW2, is a derivative of pRK 2501 carrying the Escherichia coli lac operon without a promoter, downstream of a polylinker sequence. We have cloned a number of DNA fragments carrying promoters into this plasmid and measured promoter activity in both E. coli and Pseudomonas aeruginosa. Promoters carrying consensus -10 and -35 sequences were active in both backgrounds and the dependence of activity on the nucleotide sequence of the 35 region was the same in both cases. We also measured the activity of two promoters at which transcription in E. coli was totally dependent on the E. coli activators CRP and FNR: both promoters were found to be active in P. aeruginosa.
AB - The broad-host-range plasmid, pRW2, is a derivative of pRK 2501 carrying the Escherichia coli lac operon without a promoter, downstream of a polylinker sequence. We have cloned a number of DNA fragments carrying promoters into this plasmid and measured promoter activity in both E. coli and Pseudomonas aeruginosa. Promoters carrying consensus -10 and -35 sequences were active in both backgrounds and the dependence of activity on the nucleotide sequence of the 35 region was the same in both cases. We also measured the activity of two promoters at which transcription in E. coli was totally dependent on the E. coli activators CRP and FNR: both promoters were found to be active in P. aeruginosa.
UR - https://www.scopus.com/pages/publications/0025707308
M3 - Article
C2 - 2109722
SN - 0378-1097
VL - 55
SP - 221
EP - 225
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1-2
ER -