Comparison of plant cell turgor pressure measurement by pressure probe and micromanipulation

L Wang, D Hukin, Jeremy Pritchard, C Thomas

Research output: Contribution to journalArticle

33 Citations (Scopus)


The conventional method of measuring plant cell turgor pressure is the pressure probe but applying this method to single cells in suspension culture is technically difficult and requires puncture of the cell wall. Conversely, compression testing by micromanipulation is particularly suited to studies on single cells, and can be used to characterise cell wall mechanical properties, but has not been used to measure turgor pressure. In order to demonstrate that the micromanipulation method can do this, pressure measurements by both methods were compared on single suspension-cultured tomato (Lycopersicon esculentum vf36) cells and generally were in good agreement. This validates further the micromanipulation method and demonstrates its capability to measure turgor pressure during water loss. It also suggests that it might eventually be used to estimate plant cell hydraulic conductivity.
Original languageEnglish
Pages (from-to)1147-1150
Number of pages4
JournalBiotechnology Letters
Issue number15
Publication statusPublished - 1 Aug 2006


  • tomato
  • turgor
  • micromanipulation
  • pressure probe


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