Comparison of multiple electrode aggregometry with lumi-aggregometry for the diagnosis of patients with mild bleeding disorders: Comparison of MEA and LTA

Rashid Hafidh Rashid Al Ghaithi; Sian Drake; Steve Watson; Neil Morgan; Paul Harrison

doi:10.1111/jth.13784

Comparison of multiple electrode aggregometry with lumi-aggregometry for the diagnosis of patients with mild bleeding disorders: Comparison of MEA and LTA

Rashid Hafidh Rashid Al Ghaithi, Sian Drake, Steve Watson, Neil Morgan, Paul Harrison

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

220 Downloads (Pure)

Abstract

Background: Multiple electrode aggregometry (MEA) measures changes in electrical impedance caused by platelet aggregation in whole blood. This approach is faster, more convenient and offers the advantage over light transmission aggregometry (LTA) of assessing platelet function in whole blood and reducing pre-analytical errors associated with preparation of platelet rich plasma (PRP). Several studies indicate the utility of this method in assessing platelet inhibition in individuals taking antiplatelet agents e.g. aspirin and clopidogrel.
Objective: Our current study sought to evaluate the ability of MEA in diagnosing patients with mild bleeding disorders by comparison with light transmission lumi-aggregometry (Lumi-LTA).
Methods: 40 healthy subjects and 109 patients with a clinical diagnosis of a mild bleeding disorder were recruited into the UK Genotyping and Phenotyping of Platelets study (GAPP, ISRCTN 77951167). MEA was performed on whole blood using one or two concentrations of ADP, PAR-1 peptide, arachidonic acid and collagen. Lumi-LTA was performed in PRP using several concentrations of ADP, adrenaline, arachidonic acid, collagen, PAR-1 peptide and ristocetin.
Results: Of 109 patients tested, 54 (49%) patients gave abnormal responses by lumi-LTA to one or more agonists. In contrast, only 16 (15%) patients were shown to have abnormal responses to one or more agonists by MEA.
Conclusions: In this study we showed that MEA is less sensitive in identifying patients with abnormal platelet function relative to lumi-LTA.

Original language	English
Pages (from-to)	2045-2052
Number of pages	8
Journal	Journal of thrombosis and haemostasis : JTH
Volume	15
Issue number	10
Early online date	13 Sept 2017
DOIs	https://doi.org/10.1111/jth.13784
Publication status	Published - Oct 2017

Keywords

Light transmission lumi-aggregometry
platelet function defects
platelet aggregation
multiple electrode aggregometry
mild bleeding disorders

Access to Document

10.1111/jth.13784

Ghaithi_et_al_Comparison_of_multiple_electrode_aggregometry_J_Thromb_Haemost_2017
This is the peer reviewed version of the following article: Al Ghaithi, R, Drake, S, Watson, SP, Morgan, NV, Harrison, P. Comparison of multiple electrode aggregometry with lumi‐aggregometry for the diagnosis of patients with mild bleeding disorders. J Thromb Haemost 2017; 15: 2045– 52., which has been published in final form at https://doi.org/10.1111/jth.13784. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Accepted author manuscript, 900 KBLicence: Other (please specify with Rights Statement)

https://onlinelibrary.wiley.com/doi/full/10.1111/jth.13784Licence: None: All rights reserved

Cite this

@article{64c8028d5f9e4464afe8d826682e8ffa,

title = "Comparison of multiple electrode aggregometry with lumi-aggregometry for the diagnosis of patients with mild bleeding disorders: Comparison of MEA and LTA",

abstract = "Background: Multiple electrode aggregometry (MEA) measures changes in electrical impedance caused by platelet aggregation in whole blood. This approach is faster, more convenient and offers the advantage over light transmission aggregometry (LTA) of assessing platelet function in whole blood and reducing pre-analytical errors associated with preparation of platelet rich plasma (PRP). Several studies indicate the utility of this method in assessing platelet inhibition in individuals taking antiplatelet agents e.g. aspirin and clopidogrel. Objective: Our current study sought to evaluate the ability of MEA in diagnosing patients with mild bleeding disorders by comparison with light transmission lumi-aggregometry (Lumi-LTA). Methods: 40 healthy subjects and 109 patients with a clinical diagnosis of a mild bleeding disorder were recruited into the UK Genotyping and Phenotyping of Platelets study (GAPP, ISRCTN 77951167). MEA was performed on whole blood using one or two concentrations of ADP, PAR-1 peptide, arachidonic acid and collagen. Lumi-LTA was performed in PRP using several concentrations of ADP, adrenaline, arachidonic acid, collagen, PAR-1 peptide and ristocetin. Results: Of 109 patients tested, 54 (49%) patients gave abnormal responses by lumi-LTA to one or more agonists. In contrast, only 16 (15%) patients were shown to have abnormal responses to one or more agonists by MEA. Conclusions: In this study we showed that MEA is less sensitive in identifying patients with abnormal platelet function relative to lumi-LTA. ",

keywords = "Light transmission lumi-aggregometry, platelet function defects, platelet aggregation, multiple electrode aggregometry, mild bleeding disorders",

author = "{Al Ghaithi}, {Rashid Hafidh Rashid} and Sian Drake and Steve Watson and Neil Morgan and Paul Harrison",

year = "2017",

month = oct,

doi = "10.1111/jth.13784",

language = "English",

volume = "15",

pages = "2045--2052",

journal = "Journal of thrombosis and haemostasis : JTH",

issn = "1538-7933",

publisher = "Wiley",

number = "10",

}

Comparison of multiple electrode aggregometry with lumi-aggregometry for the diagnosis of patients with mild bleeding disorders: Comparison of MEA and LTA. / Al Ghaithi, Rashid Hafidh Rashid; Drake, Sian; Watson, Steve et al.
In: Journal of thrombosis and haemostasis : JTH, Vol. 15, No. 10, 10.2017, p. 2045-2052.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Comparison of multiple electrode aggregometry with lumi-aggregometry for the diagnosis of patients with mild bleeding disorders

T2 - Comparison of MEA and LTA

AU - Al Ghaithi, Rashid Hafidh Rashid

AU - Drake, Sian

AU - Watson, Steve

AU - Morgan, Neil

AU - Harrison, Paul

PY - 2017/10

Y1 - 2017/10

N2 - Background: Multiple electrode aggregometry (MEA) measures changes in electrical impedance caused by platelet aggregation in whole blood. This approach is faster, more convenient and offers the advantage over light transmission aggregometry (LTA) of assessing platelet function in whole blood and reducing pre-analytical errors associated with preparation of platelet rich plasma (PRP). Several studies indicate the utility of this method in assessing platelet inhibition in individuals taking antiplatelet agents e.g. aspirin and clopidogrel. Objective: Our current study sought to evaluate the ability of MEA in diagnosing patients with mild bleeding disorders by comparison with light transmission lumi-aggregometry (Lumi-LTA). Methods: 40 healthy subjects and 109 patients with a clinical diagnosis of a mild bleeding disorder were recruited into the UK Genotyping and Phenotyping of Platelets study (GAPP, ISRCTN 77951167). MEA was performed on whole blood using one or two concentrations of ADP, PAR-1 peptide, arachidonic acid and collagen. Lumi-LTA was performed in PRP using several concentrations of ADP, adrenaline, arachidonic acid, collagen, PAR-1 peptide and ristocetin. Results: Of 109 patients tested, 54 (49%) patients gave abnormal responses by lumi-LTA to one or more agonists. In contrast, only 16 (15%) patients were shown to have abnormal responses to one or more agonists by MEA. Conclusions: In this study we showed that MEA is less sensitive in identifying patients with abnormal platelet function relative to lumi-LTA.

AB - Background: Multiple electrode aggregometry (MEA) measures changes in electrical impedance caused by platelet aggregation in whole blood. This approach is faster, more convenient and offers the advantage over light transmission aggregometry (LTA) of assessing platelet function in whole blood and reducing pre-analytical errors associated with preparation of platelet rich plasma (PRP). Several studies indicate the utility of this method in assessing platelet inhibition in individuals taking antiplatelet agents e.g. aspirin and clopidogrel. Objective: Our current study sought to evaluate the ability of MEA in diagnosing patients with mild bleeding disorders by comparison with light transmission lumi-aggregometry (Lumi-LTA). Methods: 40 healthy subjects and 109 patients with a clinical diagnosis of a mild bleeding disorder were recruited into the UK Genotyping and Phenotyping of Platelets study (GAPP, ISRCTN 77951167). MEA was performed on whole blood using one or two concentrations of ADP, PAR-1 peptide, arachidonic acid and collagen. Lumi-LTA was performed in PRP using several concentrations of ADP, adrenaline, arachidonic acid, collagen, PAR-1 peptide and ristocetin. Results: Of 109 patients tested, 54 (49%) patients gave abnormal responses by lumi-LTA to one or more agonists. In contrast, only 16 (15%) patients were shown to have abnormal responses to one or more agonists by MEA. Conclusions: In this study we showed that MEA is less sensitive in identifying patients with abnormal platelet function relative to lumi-LTA.

KW - Light transmission lumi-aggregometry

KW - platelet function defects

KW - platelet aggregation

KW - multiple electrode aggregometry

KW - mild bleeding disorders

U2 - 10.1111/jth.13784

DO - 10.1111/jth.13784

M3 - Article

SN - 1538-7933

VL - 15

SP - 2045

EP - 2052

JO - Journal of thrombosis and haemostasis : JTH

JF - Journal of thrombosis and haemostasis : JTH

IS - 10

ER -

Comparison of multiple electrode aggregometry with lumi-aggregometry for the diagnosis of patients with mild bleeding disorders: Comparison of MEA and LTA

Abstract

Keywords

Access to Document

Fingerprint

Cite this