Comparison of bone marrow cell growth on 2D and 3D alginate hydrogels

Jake Barralet, Lan Wang, MA Lawson, JT Triffitt, Paul Cooper, Richard Shelton

Research output: Contribution to journalArticle

91 Citations (Scopus)


Calcium cross-linked sodium alginate hydrogels have several advantageous features making them potentially suitable as tissue engineering scaffolds and this material has been previously used in many biomedical applications. 3D cell culture systems are often very different from 2D petri dish type cultures. in this study the effect of alginate hydrogel architecture was investigated by comparing rat bone marrow cell proliferation and differentiation on calcium cross linked sodium alginate discs and 1mm internal diameter tubes. It was found that bone marrow cell proliferation was diminished as the concentration of alginate in the 2D hydrogel substrates increased, yet proliferation was extensive on tubular alginate constructs with high alginate contents. Alginate gel thickness was found to be an important parameter in determining cell behaviour and the different geometries did not generate significant alterations in BMC differentiation profiles. (C) 2005 Springer Science + Business Media, Inc.
Original languageEnglish
Pages (from-to)515-519
Number of pages5
JournalJournal of materials science. Materials in medicine
Issue number16
Publication statusPublished - 1 Jun 2005


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