Comparative Genomic Hybridization Detects Secondary Chromosomal Deletions in Escherichia coli K-12 MG1655 Mutants and Highlights Instability in the flhDC Region

Jonathan Hobman, Mala Patel, GA Hidalgo-Arroyo, SJL Cariss, MB Avison, Charles Penn, Chrystala Constantinidou

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The use of whole-genome microarrays for monitoring mutagenized or otherwise engineered genetic derivatives is a potentially powerful tool for checking genomic integrity. Using comparative genomic hybridization of a number of unrelated, directed deletion mutants in Escherichia coli K-12 MG1655, we identified unintended secondary genomic deletions in the flhDC region in delta fnr, delta crp, and delta creB mutants. These deletions were confirmed by PCR and phenotypic tests. Our findings show that nonmotile progeny are found in some MG1655 directed deletion mutants, and studies on the effects of gene knockouts should be viewed with caution when the mutants have not been screened for the presence of secondary deletions or confirmed by other methods.
Original languageEnglish
Pages (from-to)8786-8792
Number of pages7
JournalJournal of Bacteriology
Volume189
Issue number24
Early online date31 Aug 2007
DOIs
Publication statusPublished - 31 Aug 2007

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