Combination of immunohistochemistry and laser ablation ICP mass spectrometry for imaging of cancer biomarkers

J Seuma, Josephine Bunch, A Cox, C McLeod, J Bell, C Murray

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Laser ablation (LA) ICP-MS has been developed as a new tool for imaging of cancer biomarkers in tissue sections. The distribution of two breast cancer-associated proteins, MUC-1 and HER2 was studied based on multiple line rastering of tissue sections and measurement of relevant Au/Ag tagged antibodies bound to the tissue. Comparisons with optical microscopy indicated extremely high sensitivity for the LA technique and sufficiently good resolution to permit fine scale feature mapping at the cellular level. Application to the quantitative assessment of HER2 expression in tissue microarrays was demonstrated.
Original languageEnglish
Pages (from-to)3775-3784
Number of pages10
JournalProteomics
Volume8
Issue number18
DOIs
Publication statusPublished - 1 Sep 2008

Keywords

  • HER2
  • elemental (Au/Ag) tagged antibodies
  • laser ablation ICP mass spectrometry
  • cellular imaging
  • cancer biomarkers

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