Colony counting is a major source of variation in CFU-GM results between centres

M A Lumley; R Burgess; L J Billingham; D F McDonald; D W Milligan

Colony counting is a major source of variation in CFU-GM results between centres

M A Lumley, R Burgess, L J Billingham, D F McDonald, D W Milligan

Research output: Contribution to journal › Article › peer-review

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Abstract

The results for colony forming unit granulocyte-macrophage (CFU-GM) assays vary substantially between centres. It is possible that colony counting is largely responsible for this discrepancy. In order to examine this exclusively from the many factors that make up the CFU-GM assay, we performed a colony counting exercise involving 11 laboratories. Two-way analysis of variance showed a highly significant difference (P = 0.0001) in the counts obtained from the centres. One centre was found to score consistently high and two others scored consistently low numbers of colonies. This suggests that identification of colonies is a major source of variation between centres.

Original language	English
Pages (from-to)	481-4
Number of pages	4
Journal	British Journal of Haematology
Volume	97
Issue number	2
Publication status	Published - May 1997

Keywords

Analysis of Variance
Colony-Forming Units Assay
Granulocytes
Hematologic Neoplasms
Hematopoietic Stem Cells
Humans
Macrophages
Reference Values

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title = "Colony counting is a major source of variation in CFU-GM results between centres",

abstract = "The results for colony forming unit granulocyte-macrophage (CFU-GM) assays vary substantially between centres. It is possible that colony counting is largely responsible for this discrepancy. In order to examine this exclusively from the many factors that make up the CFU-GM assay, we performed a colony counting exercise involving 11 laboratories. Two-way analysis of variance showed a highly significant difference (P = 0.0001) in the counts obtained from the centres. One centre was found to score consistently high and two others scored consistently low numbers of colonies. This suggests that identification of colonies is a major source of variation between centres.",

keywords = "Analysis of Variance, Colony-Forming Units Assay, Granulocytes, Hematologic Neoplasms, Hematopoietic Stem Cells, Humans, Macrophages, Reference Values",

author = "Lumley, {M A} and R Burgess and Billingham, {L J} and McDonald, {D F} and Milligan, {D W}",

year = "1997",

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language = "English",

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pages = "481--4",

journal = "British Journal of Haematology",

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T1 - Colony counting is a major source of variation in CFU-GM results between centres

AU - Lumley, M A

AU - Burgess, R

AU - Billingham, L J

AU - McDonald, D F

AU - Milligan, D W

PY - 1997/5

Y1 - 1997/5

N2 - The results for colony forming unit granulocyte-macrophage (CFU-GM) assays vary substantially between centres. It is possible that colony counting is largely responsible for this discrepancy. In order to examine this exclusively from the many factors that make up the CFU-GM assay, we performed a colony counting exercise involving 11 laboratories. Two-way analysis of variance showed a highly significant difference (P = 0.0001) in the counts obtained from the centres. One centre was found to score consistently high and two others scored consistently low numbers of colonies. This suggests that identification of colonies is a major source of variation between centres.

AB - The results for colony forming unit granulocyte-macrophage (CFU-GM) assays vary substantially between centres. It is possible that colony counting is largely responsible for this discrepancy. In order to examine this exclusively from the many factors that make up the CFU-GM assay, we performed a colony counting exercise involving 11 laboratories. Two-way analysis of variance showed a highly significant difference (P = 0.0001) in the counts obtained from the centres. One centre was found to score consistently high and two others scored consistently low numbers of colonies. This suggests that identification of colonies is a major source of variation between centres.

KW - Analysis of Variance

KW - Colony-Forming Units Assay

KW - Granulocytes

KW - Hematologic Neoplasms

KW - Hematopoietic Stem Cells

KW - Humans

KW - Macrophages

KW - Reference Values

M3 - Article

C2 - 9163619

SN - 0007-1048

VL - 97

SP - 481

EP - 484

JO - British Journal of Haematology

JF - British Journal of Haematology

IS - 2

ER -

Colony counting is a major source of variation in CFU-GM results between centres

Abstract

Keywords

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