Collection and Preparation of Clinical Samples for Metabolomics.

Warwick Dunn; Andrew Chetwynd; Giovanny Rodriguez Blanco

doi:10.1007/978-3-319-47656-8_2

Collection and Preparation of Clinical Samples for Metabolomics.

Warwick Dunn, Andrew Chetwynd, Giovanny Rodriguez Blanco

Biosciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

19 Citations (Scopus)

Abstract

A wide range of biofluids (urine, serum, plasma, saliva, etc.) as well as cellular and tissue samples can be collected and investigated in clinical metabolomic studies. The choice of sample is dependent on the clinical question being investigated with biofluids typically studied to identify biomarkers, whereas tissues and primary/immortalised cells are typically studied to investigate mechanisms associated with pathophysiological processes. Methods applied to collect samples, quench metabolism and extract samples differ between sample types from simple collect, dilute and analyse methods for urine to complex washing, quenching and biphasic extraction methods for tissues. The range of sample collection and extraction methods are discussed with sample-specific considerations highlighted. Finally, methods for imaging of cells and tissues and for in vivo metabolomic analysis will also be introduced.

Original language	English
Title of host publication	Metabolomics: From Fundamentals to Clinical Applications
Publisher	Springer
Pages	19
Number of pages	44
ISBN (Electronic)	9783319476568
ISBN (Print)	9783319476551
DOIs	https://doi.org/10.1007/978-3-319-47656-8_2
Publication status	Published - 1 Feb 2017

Publication series

Name	Advances in Experimental Medicine and Biology
Publisher	Springer
Volume	965

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AU - Rodriguez Blanco, Giovanny

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BT - Metabolomics: From Fundamentals to Clinical Applications

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Collection and Preparation of Clinical Samples for Metabolomics.

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