Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death

S Hosseinzadeh; Ian Brewis; A Eley; A Pacey

doi:10.1093/humrep/16.2.293

Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death

S Hosseinzadeh, Ian Brewis, A Eley, A Pacey

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Abstract

The aim of this work was to investigate the effect of elementary bodies (EB) of Chlamydia trachomatis serovars E and LGV on sperm motility, viability and acrosomal status. Highly motile preparations of spermatozoa from normozoospermic patients were co-incubated for 6 h with 0.54x10(6) EB per ml. At 1, 3 and 6 h of incubation, sperm motility was determined by computer-assisted semen analysis (CASA) and the proportion of dead cells determined by the hypo-osmotic swelling (HOS) test. Acrosomal status was also examined using a standard monoclonal antibody assay. In the absence of EB, the percentage of motile spermatozoa remained >69% over the 6h incubation and the proportion of dead spermatozoa at

Original language	English
Pages (from-to)	293-299
Number of pages	7
Journal	Human Reproduction
Volume	16
Issue number	2
DOIs	https://doi.org/10.1093/humrep/16.2.293
Publication status	Published - 1 Feb 2001

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title = "Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death",

abstract = "The aim of this work was to investigate the effect of elementary bodies (EB) of Chlamydia trachomatis serovars E and LGV on sperm motility, viability and acrosomal status. Highly motile preparations of spermatozoa from normozoospermic patients were co-incubated for 6 h with 0.54x10(6) EB per ml. At 1, 3 and 6 h of incubation, sperm motility was determined by computer-assisted semen analysis (CASA) and the proportion of dead cells determined by the hypo-osmotic swelling (HOS) test. Acrosomal status was also examined using a standard monoclonal antibody assay. In the absence of EB, the percentage of motile spermatozoa remained >69\% over the 6h incubation and the proportion of dead spermatozoa at ",

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T1 - Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death

AU - Hosseinzadeh, S

AU - Brewis, Ian

AU - Eley, A

AU - Pacey, A

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AB - The aim of this work was to investigate the effect of elementary bodies (EB) of Chlamydia trachomatis serovars E and LGV on sperm motility, viability and acrosomal status. Highly motile preparations of spermatozoa from normozoospermic patients were co-incubated for 6 h with 0.54x10(6) EB per ml. At 1, 3 and 6 h of incubation, sperm motility was determined by computer-assisted semen analysis (CASA) and the proportion of dead cells determined by the hypo-osmotic swelling (HOS) test. Acrosomal status was also examined using a standard monoclonal antibody assay. In the absence of EB, the percentage of motile spermatozoa remained >69% over the 6h incubation and the proportion of dead spermatozoa at

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Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death

Abstract

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