Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death

S Hosseinzadeh, Ian Brewis, A Eley, A Pacey

    Research output: Contribution to journalArticle

    69 Citations (Scopus)

    Abstract

    The aim of this work was to investigate the effect of elementary bodies (EB) of Chlamydia trachomatis serovars E and LGV on sperm motility, viability and acrosomal status. Highly motile preparations of spermatozoa from normozoospermic patients were co-incubated for 6 h with 0.54x10(6) EB per ml. At 1, 3 and 6 h of incubation, sperm motility was determined by computer-assisted semen analysis (CASA) and the proportion of dead cells determined by the hypo-osmotic swelling (HOS) test. Acrosomal status was also examined using a standard monoclonal antibody assay. In the absence of EB, the percentage of motile spermatozoa remained >69% over the 6h incubation and the proportion of dead spermatozoa at
    Original languageEnglish
    Pages (from-to)293-299
    Number of pages7
    JournalHuman Reproduction
    Volume16
    Issue number2
    DOIs
    Publication statusPublished - 1 Feb 2001

    Fingerprint

    Dive into the research topics of 'Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death'. Together they form a unique fingerprint.

    Cite this