Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase

J M Lodge; S D Minchin; L J Piddock; S J Busby

Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase

J M Lodge, S D Minchin, L J Piddock, S J Busby

Research output: Contribution to journal › Article › peer-review

91 Citations (Scopus)

Abstract

The chromosomal gene from Pseudomonas aeruginosa encoding beta-lactamase has been cloned, and the sequence determined and compared with corresponding sequences of beta-lactamases from members of the enterobacteriaceae. Upstream of the beta-lactamase gene is an open reading frame which we postulate encodes a regulatory protein, AmpR. We identified a helix-turn-helix region in AmpR and a putative AmpR-binding site.

Original language	English
Pages (from-to)	627-31
Number of pages	5
Journal	Biochemical Journal
Volume	272
Issue number	3
Publication status	Published - 15 Dec 1990

Keywords

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Genes, Bacterial
Genes, Regulator
Molecular Sequence Data
Open Reading Frames
Pseudomonas aeruginosa
Restriction Mapping
Sequence Homology, Nucleic Acid
beta-Lactamases

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title = "Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase",

abstract = "The chromosomal gene from Pseudomonas aeruginosa encoding beta-lactamase has been cloned, and the sequence determined and compared with corresponding sequences of beta-lactamases from members of the enterobacteriaceae. Upstream of the beta-lactamase gene is an open reading frame which we postulate encodes a regulatory protein, AmpR. We identified a helix-turn-helix region in AmpR and a putative AmpR-binding site.",

keywords = "Amino Acid Sequence, Base Sequence, Cloning, Molecular, Genes, Bacterial, Genes, Regulator, Molecular Sequence Data, Open Reading Frames, Pseudomonas aeruginosa, Restriction Mapping, Sequence Homology, Nucleic Acid, beta-Lactamases",

author = "Lodge, {J M} and Minchin, {S D} and Piddock, {L J} and Busby, {S J}",

year = "1990",

month = dec,

day = "15",

language = "English",

volume = "272",

pages = "627--31",

journal = "Biochemical Journal",

issn = "0264-6021",

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TY - JOUR

T1 - Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase

AU - Lodge, J M

AU - Minchin, S D

AU - Piddock, L J

AU - Busby, S J

PY - 1990/12/15

Y1 - 1990/12/15

N2 - The chromosomal gene from Pseudomonas aeruginosa encoding beta-lactamase has been cloned, and the sequence determined and compared with corresponding sequences of beta-lactamases from members of the enterobacteriaceae. Upstream of the beta-lactamase gene is an open reading frame which we postulate encodes a regulatory protein, AmpR. We identified a helix-turn-helix region in AmpR and a putative AmpR-binding site.

AB - The chromosomal gene from Pseudomonas aeruginosa encoding beta-lactamase has been cloned, and the sequence determined and compared with corresponding sequences of beta-lactamases from members of the enterobacteriaceae. Upstream of the beta-lactamase gene is an open reading frame which we postulate encodes a regulatory protein, AmpR. We identified a helix-turn-helix region in AmpR and a putative AmpR-binding site.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cloning, Molecular

KW - Genes, Bacterial

KW - Genes, Regulator

KW - Molecular Sequence Data

KW - Open Reading Frames

KW - Pseudomonas aeruginosa

KW - Restriction Mapping

KW - Sequence Homology, Nucleic Acid

KW - beta-Lactamases

M3 - Article

C2 - 2125210

SN - 0264-6021

VL - 272

SP - 627

EP - 631

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase

Abstract

Keywords

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