TY - JOUR
T1 - Cleavage of beta-carotene as the first step in sexual hormone synthesis in zygomycetes is mediated by a trisporic acid regulated beta-carotene oxygenase
AU - Burmester, Anke
AU - Richter, Mareike
AU - Schultze, Kornelia
AU - Voelz, Kerstin
AU - Schachtschabel, Doreen
AU - Boland, Wilhelm
AU - Wöstemeyer, Johannes
AU - Schimek, Christine
PY - 2007/11
Y1 - 2007/11
N2 - Carotene cleavage is the necessary initial step in the biosynthesis of trisporic acid, the sexual signal in zygomycete fungi. Two genes encoding putative carotene oxygenases, designated tsp3 and tsp4, were identified in the genome of the zygomycete Rhizopus oryzae. Using heterologous primers, tsp3 was cloned and sequenced also from Blakeslea trispora. tsp3 transcription correlates with sexual development in both species. Northern hybridization of B. trispora mRNA revealed strong induction of tsp3 transcription in mated cultures. A very strong and direct transient induction of transcription by trisporic acid was proven by quantitative real-time PCR analysis. In R. oryzae, transcriptional induction is also inducible by stimulation with trisporoids and depends on the developmental stage of the mycelium. The functionality of the tsp3 gene product as carotene cleavage enzyme was shown as loss of carotene in an Escherichia coli strain transformed to carotene production and tsp3 expression.
AB - Carotene cleavage is the necessary initial step in the biosynthesis of trisporic acid, the sexual signal in zygomycete fungi. Two genes encoding putative carotene oxygenases, designated tsp3 and tsp4, were identified in the genome of the zygomycete Rhizopus oryzae. Using heterologous primers, tsp3 was cloned and sequenced also from Blakeslea trispora. tsp3 transcription correlates with sexual development in both species. Northern hybridization of B. trispora mRNA revealed strong induction of tsp3 transcription in mated cultures. A very strong and direct transient induction of transcription by trisporic acid was proven by quantitative real-time PCR analysis. In R. oryzae, transcriptional induction is also inducible by stimulation with trisporoids and depends on the developmental stage of the mycelium. The functionality of the tsp3 gene product as carotene cleavage enzyme was shown as loss of carotene in an Escherichia coli strain transformed to carotene production and tsp3 expression.
KW - Fatty Acids, Unsaturated
KW - Fungal Proteins
KW - Fungi
KW - Gene Expression Regulation, Fungal
KW - Oxygenases
KW - Polymerase Chain Reaction
KW - RNA, Messenger
KW - Transcription, Genetic
KW - beta Carotene
U2 - 10.1016/j.fgb.2007.07.008
DO - 10.1016/j.fgb.2007.07.008
M3 - Article
C2 - 17822929
SN - 1096-0937
VL - 44
SP - 1096
EP - 1108
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
IS - 11
ER -