Circulating tumour DNA sequencing to determine therapeutic response and identify tumour heterogeneity in patients with paediatric solid tumours

Reda Stankunaite, Sally L. George, Lewis Gallagher, Sabri Jamal, Ridwan Shaikh, Lina Yuan, Debbie Hughes, Paula Z. Proszek, Paul Carter, Grzegorz Pietka, Timon Heide, Chela James, Haider Tari, Claire Lynn, Neha Jain, Laura Rey Portela, Tony Rogers, Sucheta J. Vaidya, Julia C. Chisholm, Fernando CarcellerElwira Szychot, Henry Mandeville, Paola Angelini, Angela B. Jesudason, Michael Jackson, Lynley V. Marshall, Susanne A. Gatz, John Anderson, Andrea Sottoriva, Louis Chesler*, Michael Hubank*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Objective
Clinical diagnostic sequencing of circulating tumour DNA (ctDNA) is well advanced for adult patients, but application to paediatric cancer patients lags behind.
Methods
To address this, we have developed a clinically relevant (67 gene) NGS capture panel and accompanying workflow that enables sensitive and reliable detection of low-frequency genetic variants in cell-free DNA (cfDNA) from children with solid tumours. We combined gene panel sequencing with low pass whole-genome sequencing of the same library to inform on genome-wide copy number changes in the blood.
Results
Analytical validity was evaluated using control materials, and the method was found to be highly sensitive (0.96 for SNVs and 0.97 for INDEL), specific (0.82 for SNVs and 0.978 for INDEL), repeatable (>0.93 [95% CI: 0.89–0.95]) and reproducible (>0.87 [95% CI: 0.87–0.95]). Potential for clinical application was demonstrated in 39 childhood cancer patients with a spectrum of solid tumours in which the single nucleotide variants expected from tumour sequencing were detected in cfDNA in 94.4% (17/18) of cases with active extracranial disease. In 13 patients, where serial samples were available, we show a close correlation between events detected in cfDNA and treatment response, demonstrate that cfDNA analysis could be a useful tool to monitor disease progression, and show cfDNA sequencing has the potential to identify targetable variants that were not detected in tumour samples.
Conclusions
This is the first pan-cancer DNA sequencing panel that we know to be optimised for cfDNA in children for blood-based molecular diagnostics in paediatric solid tumours.
Original languageEnglish
Pages (from-to)209-220
Number of pages12
JournalEuropean Journal of Cancer
Volume162
Early online date18 Dec 2021
DOIs
Publication statusPublished - Feb 2022

Bibliographical note

Publisher Copyright:
© 2021 The Authors

Keywords

  • Cancer heterogeneity
  • Cell-free DNA
  • Clinical targeted sequencing
  • ctDNA
  • Liquid biopsy
  • Paediatric oncology
  • Personalised medicine

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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