Circulating form of human vascular adhesion protein-1 (VAP-1): increased serum levels in inflammatory liver diseases

R Kurkijärvi, R Leino, T Möttönen, S Jalkanen, M Salmi, David Adams

Research output: Contribution to journalArticlepeer-review

137 Citations (Scopus)


Vascular adhesion protein-1 (VAP-1) is a dimeric 170-kDa endothelial transmembrane molecule that under normal conditions is most strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. It is a glycoprotein that mediates tissue-selective lymphocyte adhesion in a sialic acid-dependent manner. In this study, we report the detection of a soluble form of VAP-1 in circulation. We developed a quantitative sandwich ELISA using novel anti-VAP-1 mAbs and used it to determine the levels of soluble VAP-1 (sVAP-1) in the serum of healthy individuals and in patients with inflammatory diseases. In healthy persons, circulating sVAP-1 concentrations were 49 to 138 ng/ml. Immunoblotting studies revealed that the apparent molecular mass of dimeric sVAP-1 is slightly (approximately 10 kDa) higher than that of transmembrane VAP-1 under nonreducing conditions. In contrast, the electrophoretic mobilities of monomeric sVAP-1 and transmembrane VAP-1 were similar after reduction and boiling. Adhesion assays showed that the circulating sVAP-1 modulates lymphocyte binding to endothelial cells. Inflammation can cause an elevation of serum sVAP-1 levels, because sVAP-1 concentrations in patients with certain liver diseases were two- to fourfold higher than those in normal individuals. In contrast, rheumatoid arthritis and inflammatory bowel diseases were not associated with elevated levels of sVAP-1. These findings indicate that there is a functionally active, soluble form of VAP-1 in circulation and suggest that the serum level of sVAP-1 might be a useful marker of disease activity in inflammatory liver diseases.
Original languageEnglish
Pages (from-to)1549-57
Number of pages9
JournalJournal of Immunology
Issue number3
Publication statusPublished - 1 Aug 1998


  • Adult
  • Aged
  • Amine Oxidase (Copper-Containing)
  • Carcinoma, Hepatocellular
  • Cell Adhesion
  • Cell Adhesion Molecules
  • Cholangitis, Sclerosing
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Liver Cirrhosis, Biliary
  • Liver Diseases
  • Liver Diseases, Alcoholic
  • Liver Neoplasms
  • Male
  • Middle Aged
  • Solubility
  • Up-Regulation


Dive into the research topics of 'Circulating form of human vascular adhesion protein-1 (VAP-1): increased serum levels in inflammatory liver diseases'. Together they form a unique fingerprint.

Cite this