Chemical force microscopy with active enzymes

M Fiorini, R McKendry, MA Cooper, Trevor Rayment, C Abell

Research output: Contribution to journalArticle

26 Citations (Scopus)


The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with competitive binding of other ligands in solution show that the observed adhesion forces arise predominantly from specific interactions between the immobilized enzyme and surface-bound adenine derivative. These experiments represent a step in the development of a screening methodology based upon chemical force microscopy.
Original languageEnglish
Pages (from-to)2471-2476
Number of pages6
JournalBiophysical Journal
Publication statusPublished - 1 Jan 2001


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