Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Josefine Hirschfeld; Ilaria Chicca; Carolyn G.J. Moonen; Phillipa White; Martin Ling; Helen Wright; Paul R. Cooper; Michael Milward; Iain Chapple

doi:10.1007/978-1-0716-2780-8_27

Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Josefine Hirschfeld
, Ilaria Chicca
, Carolyn G.J. Moonen
, Phillipa White
, Martin Ling
, Helen Wright
, Paul R. Cooper
, Michael Milward
, Iain Chapple

Dentistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils as well as of oral neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.

Original language	English
Title of host publication	Oral Biology
Subtitle of host publication	Molecular Techniques and Applications
Publisher	Springer
Pages	451–472
Edition	3
ISBN (Electronic)	9781071627808
ISBN (Print)	9781071627792, 9781071627822
DOIs	https://doi.org/10.1007/978-1-0716-2780-8_27
Publication status	Published - 24 Nov 2022

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
Volume	2588
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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10.1007/978-1-0716-2780-8_27Licence: None: All rights reserved

1 Chapter

Correction to: Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps
Hirschfeld, J., Chicca, I. J., Moonen, C. G. J., White, P. C., Ling, M. R., Wright, H. J., Cooper, P. R., Milward, M. R. & Chapple, I. L. C., 10 Jan 2022, Oral Biology: Molecular Techniques and Applications. Seymour, G. J., Cullinan, M. P., Heng, N. C. K. & Cooper, P. R. (eds.). 3 ed. New York: Humana Press, p. C1-C1 1 p. (Methods in Molecular Biology; vol. 2588).
Research output: Chapter in Book/Report/Conference proceeding › Chapter

Cite this

Hirschfeld, J., Chicca, I., Moonen, C. G. J., White, P., Ling, M., Wright, H., Cooper, P. R., Milward, M., & Chapple, I. (2022). Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps. In Oral Biology: Molecular Techniques and Applications (3 ed., pp. 451–472). (Methods in Molecular Biology; Vol. 2588). Springer. https://doi.org/10.1007/978-1-0716-2780-8_27

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abstract = "Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils as well as of oral neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.",

author = "Josefine Hirschfeld and Ilaria Chicca and Moonen, \{Carolyn G.J.\} and Phillipa White and Martin Ling and Helen Wright and Cooper, \{Paul R.\} and Michael Milward and Iain Chapple",

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Hirschfeld, J, Chicca, I, Moonen, CGJ, White, P, Ling, M, Wright, H, Cooper, PR, Milward, M & Chapple, I 2022, Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps. in Oral Biology: Molecular Techniques and Applications. 3 edn, Methods in Molecular Biology, vol. 2588, Springer, pp. 451–472. https://doi.org/10.1007/978-1-0716-2780-8_27

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AU - Ling, Martin

AU - Wright, Helen

AU - Cooper, Paul R.

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AU - Chapple, Iain

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Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

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Correction to: Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

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