Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Phillipa White, Ilaria Chicca, Martin Ling, Helen Wright, Paul Cooper, Michael Milward, Iain Chapple

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

11 Citations (Scopus)


Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.

Original languageEnglish
Title of host publicationOral biology
Subtitle of host publicationmolecular techniques and applications
EditorsGregory J. Seymour, Mary P. Cullinan, Nicholas C. K. Heng
Number of pages17
ISBN (Electronic)978-1-4939-6685-1
ISBN (Print)978-1-4939-6683-7
Publication statusPublished - 7 Dec 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Journal Article
  • Neutrophil extracellular traps
  • Reactive oxygen species
  • Fluorescence microscopy
  • SEM
  • Chemiluminescence
  • DNA
  • Elastase
  • Myeloperoxidase
  • Cathepsin G
  • Immunostaining


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