Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Phillipa White; Ilaria Chicca; Martin Ling; Helen Wright; Paul Cooper; Michael Milward; Iain Chapple

doi:10.1007/978-1-4939-6685-1_29

Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Phillipa White, Ilaria Chicca, Martin Ling, Helen Wright, Paul Cooper, Michael Milward, Iain Chapple

Dentistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

11 Citations (Scopus)

Abstract

Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.

Original language	English
Title of host publication	Oral biology
Subtitle of host publication	molecular techniques and applications
Editors	Gregory J. Seymour, Mary P. Cullinan, Nicholas C. K. Heng
Publisher	Springer
Pages	481-497
Number of pages	17
Volume	1537
ISBN (Electronic)	978-1-4939-6685-1
ISBN (Print)	978-1-4939-6683-7
DOIs	https://doi.org/10.1007/978-1-4939-6685-1_29
Publication status	Published - 7 Dec 2016

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
Volume	1537
ISSN (Print)	1064-3745

Keywords

Journal Article
Neutrophil extracellular traps
Reactive oxygen species
Fluorescence microscopy
SEM
Chemiluminescence
DNA
Elastase
Myeloperoxidase
Cathepsin G
Immunostaining

Access to Document

10.1007/978-1-4939-6685-1_29Licence: None: All rights reserved

https://link.springer.com/protocol/10.1007%2F978-1-4939-6685-1_29Licence: None: All rights reserved

Cite this

White, P., Chicca, I., Ling, M., Wright, H., Cooper, P., Milward, M., & Chapple, I. (2016). Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps. In G. J. Seymour, M. P. Cullinan, & N. C. K. Heng (Eds.), Oral biology: molecular techniques and applications (Vol. 1537, pp. 481-497). (Methods in Molecular Biology; Vol. 1537). Springer. https://doi.org/10.1007/978-1-4939-6685-1_29

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abstract = "Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.",

keywords = "Journal Article, Neutrophil extracellular traps , Reactive oxygen species , Fluorescence microscopy , SEM , Chemiluminescence , DNA , Elastase , Myeloperoxidase , Cathepsin G , Immunostaining",

author = "Phillipa White and Ilaria Chicca and Martin Ling and Helen Wright and Paul Cooper and Michael Milward and Iain Chapple",

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White, P, Chicca, I, Ling, M, Wright, H, Cooper, P, Milward, M & Chapple, I 2016, Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps. in GJ Seymour, MP Cullinan & NCK Heng (eds), Oral biology: molecular techniques and applications. vol. 1537, Methods in Molecular Biology, vol. 1537, Springer, pp. 481-497. https://doi.org/10.1007/978-1-4939-6685-1_29

Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps. / White, Phillipa; Chicca, Ilaria; Ling, Martin et al.
Oral biology: molecular techniques and applications. ed. / Gregory J. Seymour; Mary P. Cullinan; Nicholas C. K. Heng. Vol. 1537 Springer, 2016. p. 481-497 (Methods in Molecular Biology; Vol. 1537).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

AU - White, Phillipa

AU - Chicca, Ilaria

AU - Ling, Martin

AU - Wright, Helen

AU - Cooper, Paul

AU - Milward, Michael

AU - Chapple, Iain

PY - 2016/12/7

Y1 - 2016/12/7

N2 - Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.

AB - Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.

KW - Journal Article

KW - Neutrophil extracellular traps

KW - Reactive oxygen species

KW - Fluorescence microscopy

KW - SEM

KW - Chemiluminescence

KW - DNA

KW - Elastase

KW - Myeloperoxidase

KW - Cathepsin G

KW - Immunostaining

U2 - 10.1007/978-1-4939-6685-1_29

DO - 10.1007/978-1-4939-6685-1_29

M3 - Chapter (peer-reviewed)

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SN - 978-1-4939-6683-7

VL - 1537

T3 - Methods in Molecular Biology

SP - 481

EP - 497

BT - Oral biology

A2 - Seymour, Gregory J.

A2 - Cullinan, Mary P.

A2 - Heng, Nicholas C. K.

PB - Springer

ER -

Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Abstract

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Keywords

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