Characterization of the solution properties and conformation of pneumolysin, the membrane-damaging toxin of Streptococcus pneumoniae

P J Morgan, P G Varley, A J Rowe, P W Andrew, T J Mitchell

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18 Citations (Scopus)

Abstract

Pneumolysin is a membrane-damaging toxin produced by Streptococcus pneumoniae. In order to understand fully the mode of action of this toxin, it is necessary to have an appreciation of the size, self-association behaviour and solution conformation of pneumolysin. A combination of analytical ultracentrifugation methodologies has shown that pneumolysin lacks self-association behaviour in solution and has provided a weight-average M(r) (M omega) of 52,000 +/- 2000, which was in agreement with that derived from the amino acid sequence. By determining a sedimentation coefficient (S20,w0) of 3.35 +/- 0.10 S, it was possible to suggest a model for the gross solution conformation of pneumolysin monomers. Spectroscopic methods provide additional secondary and tertiary structure information.

Original languageEnglish
Pages (from-to)671-4
Number of pages4
JournalBiochemical Journal
Volume296 ( Pt 3)
Publication statusPublished - 15 Dec 1993

Keywords

  • Amino Acids
  • Bacterial Proteins
  • Cell Membrane
  • Circular Dichroism
  • Cytotoxins
  • Protein Structure, Secondary
  • Solutions
  • Spectrometry, Fluorescence
  • Spectrophotometry, Ultraviolet
  • Streptococcus pneumoniae
  • Streptolysins
  • Ultracentrifugation

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