Abstract
The pneumolysin toxoid, Δ6 PLY, is a prototype pneumococcal protein vaccine candidate. However, its potentially detrimental residual pro-inflammatory interactions with human neutrophils are unknown. In the current study the effects of the toxoid (8-1000 ng/ml) have been compared with those of wild-type pneumolysin (WT/PLY, 8 ng/ml) on neutrophil cytosolic Ca(2+) fluxes, generation of leukotriene B(4) (LTB(4)), and release of matrix metalloproteinase-9 (MMP-9), using spectrofluorimetric, and ELISA procedures (LTB(4) and MMP-9) respectively. Exposure of neutrophils to WT/PLY resulted in influx of Ca(2+) and significant (P
Original language | English |
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Pages (from-to) | 8780-2 |
Number of pages | 3 |
Journal | Vaccine |
Volume | 29 |
Issue number | 48 |
DOIs | |
Publication status | Published - 8 Nov 2011 |
Bibliographical note
Copyright © 2011 Elsevier Ltd. All rights reserved.Keywords
- Bacterial Proteins
- Calcium
- Cells, Cultured
- Humans
- Leukotriene B4
- Matrix Metalloproteinase 9
- Neutrophil Activation
- Neutrophils
- Streptolysins
- Toxoids