Characteristics of rat liver phosphatidylinositol kinase and its presence in the plasma membrane

R. H. Michell; J. L. Harwood; R. Coleman; J. N. Hawthorne

doi:10.1016/0005-2760(67)90053-7

Characteristics of rat liver phosphatidylinositol kinase and its presence in the plasma membrane

R. H. Michell^*, J. L. Harwood, R. Coleman, J. N. Hawthorne

^*Corresponding author for this work

Biosciences

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

1. Rat liver contains a phosphatidylinositol kinase.
2. The enzyme requires phosphatidylinositol, ATP and Mg²⁺ or Mn²⁺ for activity.
3. The subcellular distribution of the enzyme does not parallel that of nuclei, mitochondria, lysosomes or endoplasmic reticulum.
4. The kinase and 5'-nucleotidase (a plasma membrane enzyme) are similary distributed in subcellular fractions.
5. The activity of phosphatidylinositol kinase is at least twice as high in purified plasma membrane as in nuclei prepared from the same crude nuclear fraction.
6. The role of the kinase at the cell surface is discussed.

Original language	English
Pages (from-to)	649-658
Number of pages	10
Journal	BBA - Lipids and Lipid Metabolism
Volume	144
Issue number	3
DOIs	https://doi.org/10.1016/0005-2760(67)90053-7
Publication status	Published - 5 Dec 1967

ASJC Scopus subject areas

Biophysics
Biochemistry
Endocrinology

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title = "Characteristics of rat liver phosphatidylinositol kinase and its presence in the plasma membrane",

abstract = "1. Rat liver contains a phosphatidylinositol kinase. 2. The enzyme requires phosphatidylinositol, ATP and Mg2+ or Mn2+ for activity. 3. The subcellular distribution of the enzyme does not parallel that of nuclei, mitochondria, lysosomes or endoplasmic reticulum. 4. The kinase and 5'-nucleotidase (a plasma membrane enzyme) are similary distributed in subcellular fractions. 5. The activity of phosphatidylinositol kinase is at least twice as high in purified plasma membrane as in nuclei prepared from the same crude nuclear fraction. 6. The role of the kinase at the cell surface is discussed.",

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AU - Michell, R. H.

AU - Harwood, J. L.

AU - Coleman, R.

AU - Hawthorne, J. N.

PY - 1967/12/5

Y1 - 1967/12/5

N2 - 1. Rat liver contains a phosphatidylinositol kinase. 2. The enzyme requires phosphatidylinositol, ATP and Mg2+ or Mn2+ for activity. 3. The subcellular distribution of the enzyme does not parallel that of nuclei, mitochondria, lysosomes or endoplasmic reticulum. 4. The kinase and 5'-nucleotidase (a plasma membrane enzyme) are similary distributed in subcellular fractions. 5. The activity of phosphatidylinositol kinase is at least twice as high in purified plasma membrane as in nuclei prepared from the same crude nuclear fraction. 6. The role of the kinase at the cell surface is discussed.

AB - 1. Rat liver contains a phosphatidylinositol kinase. 2. The enzyme requires phosphatidylinositol, ATP and Mg2+ or Mn2+ for activity. 3. The subcellular distribution of the enzyme does not parallel that of nuclei, mitochondria, lysosomes or endoplasmic reticulum. 4. The kinase and 5'-nucleotidase (a plasma membrane enzyme) are similary distributed in subcellular fractions. 5. The activity of phosphatidylinositol kinase is at least twice as high in purified plasma membrane as in nuclei prepared from the same crude nuclear fraction. 6. The role of the kinase at the cell surface is discussed.

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JF - BBA - Lipids and Lipid Metabolism

IS - 3

ER -

Characteristics of rat liver phosphatidylinositol kinase and its presence in the plasma membrane

Abstract

ASJC Scopus subject areas

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