Characteristics of an in vitro system of thymocyte positive selection

Graham Andersen; John J.T. Owen; Nel C. Moore; Eric J. Jenkinson

Characteristics of an in vitro system of thymocyte positive selection

Graham Andersen, John J.T. Owen, Nel C. Moore, Eric J. Jenkinson

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

We investigated the phenotypic changes accompanying maturation of CD4⁺CD8⁺ thymocytes in the presence of thymic stromal cells in vitro. Many of the features that are characteristic of positive selection in vivo, such as an ordered down-regulation of CD4 or CD8 together with up-regulation of the TCR-αβ complex, also occurred in vitro. Transient expression of CD69 was also observed, together with activation of IL-2 and IL-4 cytokine genes, providing further evidence that positive selection in vitro involves a pattern of gene regulation comparable to that seen in vivo.

Original language	English
Pages (from-to)	1915-1920
Number of pages	6
Journal	Journal of Immunology
Volume	153
Issue number	5
Publication status	Published - 1 Sept 1994

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Cite this

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abstract = "We investigated the phenotypic changes accompanying maturation of CD4+CD8+ thymocytes in the presence of thymic stromal cells in vitro. Many of the features that are characteristic of positive selection in vivo, such as an ordered down-regulation of CD4 or CD8 together with up-regulation of the TCR-αβ complex, also occurred in vitro. Transient expression of CD69 was also observed, together with activation of IL-2 and IL-4 cytokine genes, providing further evidence that positive selection in vitro involves a pattern of gene regulation comparable to that seen in vivo.",

author = "Graham Andersen and Owen, {John J.T.} and Moore, {Nel C.} and Jenkinson, {Eric J.}",

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AU - Owen, John J.T.

AU - Moore, Nel C.

AU - Jenkinson, Eric J.

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AB - We investigated the phenotypic changes accompanying maturation of CD4+CD8+ thymocytes in the presence of thymic stromal cells in vitro. Many of the features that are characteristic of positive selection in vivo, such as an ordered down-regulation of CD4 or CD8 together with up-regulation of the TCR-αβ complex, also occurred in vitro. Transient expression of CD69 was also observed, together with activation of IL-2 and IL-4 cytokine genes, providing further evidence that positive selection in vitro involves a pattern of gene regulation comparable to that seen in vivo.

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Characteristics of an in vitro system of thymocyte positive selection

Abstract

ASJC Scopus subject areas

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