Characterisation of the dynamic nature of lipids throughout the lifespan of genetically identical female and male Daphnia magna

Julia K. Constantinou*, Andrew D. Southam, Jouni Kvist, Martin R. Jones, Mark R. Viant, Leda Mirbahai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
159 Downloads (Pure)

Abstract

Lipids play a significant role in regulation of health and disease. To enhance our understanding of the role of lipids in regulation of lifespan and healthspan additional studies are required. Here, UHPLC-MS/MS lipidomics was used to measure dynamic changes in lipid composition as a function of age and gender in genetically identical male and female Daphnia magna with different average lifespans. We demonstrate statistically significant age-related changes in triglycerides (TG), diglycerides (DG), phosphatidylcholine, phosphatidylethanolamine, ceramide and sphingomyelin lipid groups, for example, in males, 17.04% of TG lipid species decline with age whilst 37.86% increase in relative intensity with age. In females, 23.16% decrease and 25.31% increase in relative intensity with age. Most interestingly, the rate and direction of change can differ between genetically identical female and male Daphnia magna, which could be the cause and/or the consequence of the different average lifespans between the two genetically identical genders. This study provides a benchmark dataset to understand how lipids alter as a function of age in genetically identical female and male species with different average lifespan and ageing rate.

Original languageEnglish
Article number5576
JournalScientific Reports
Volume10
DOIs
Publication statusPublished - 27 Mar 2020

Keywords

  • Aging/metabolism
  • Animals
  • Daphnia/metabolism
  • Diglycerides/metabolism
  • Female
  • Lipid Metabolism/physiology
  • Longevity/physiology
  • Male
  • Phosphatidylcholines/metabolism
  • Sphingomyelins/metabolism
  • Triglycerides/metabolism

ASJC Scopus subject areas

  • General

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