Abstract
A major factor limiting the development of non-viral gene delivery systems is the poor characterisation of polyelectrolyte complexes formed between cationic polymers and DNA. The present study uses the fluorescamine reagent to improve characterisation of poly(L-lysine) (pLL)/DNA complexes post-modified with a multivalent hydrophilic polymer by determining the availability of free amino groups. The results show that the fluorescamine reagent can be used to monitor the self-assembly reaction between pLL and DNA and the degree of surface modification of the resultant complexes with a hydrophilic polymer. This experimental approach should enable the preparation of fully defined complexes whose properties can be better related to their biological activity.
Original language | English |
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Pages (from-to) | 96-100 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 461 |
Issue number | 1-2 |
Publication status | Published - 12 Nov 1999 |
Keywords
- Animals
- Cattle
- DNA
- Dose-Response Relationship, Drug
- Ethidium
- Fluorescamine
- Fluorescent Dyes
- Genetic Therapy
- Genetic Vectors
- Hydrogen-Ion Concentration
- Kinetics
- Molecular Weight
- Nucleic Acid Conformation
- Polylysine
- Polymers
- Thymus Gland
- Time Factors