Characterisation of Nah-plasmids of IncP-9 group from natural strains of pseudomonas (in Russian)

AA Leuchuk, IM Bulyha, T Izmalkova, Yanina Sevastsyanovich, IA Kosheleva, Christopher Thomas, MA Titok

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2 Citations (Scopus)


Polymerase chain reaction studies showed that naphthalene-utilizing bacteria isolated from various localities of Belarus most often contained Nah plasmids of the P-9 incompatibility group and plasmids of indefinite systematics. The conventional incompatibility test and restriction enzyme analysis revealed three new IncP-9 subgroups: xi,eta, and IncP-9-like. In addition to the known nucleotide sequences of nahG and nahAc, two novel nahG variants were revealed by a restriction enzyme analysis of amplification products. An amplified rDNA restriction enzyme analysis (ARDRA) demonstrated that the native hosts of IncP-9 Nah plasmids were fluorescent bacteria of the genus P. seudomonas (P. fluorescens, P. putida, P. aeruginosa, and P. seudomonas sp.) and nonfluorescent bacteria of indefinite systematics.
Original languageEnglish
Pages (from-to)750-757
Number of pages8
JournalMolecular Biology
Publication statusPublished - 1 Oct 2006


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