Changes in the serum proteome associated with the development of hepatocellular carcinoma in hepatitis C-related cirrhosis

Douglas Ward, Yaping Cheng, G N'Kontchou, TT Thar, N Barget, Wenbin Wei, Lucinda Billingham, Ashley Martin, M Beaugrand, Philip Johnson

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Early diagnosis of hepatocellular carcinoma (HCC) is the key to the delivery of effective therapies. The conventional serological diagnostic test, estimation of serum alpha-fetoprotein (AFP) lacks both sensitivity and specificity as a screening tool and improved tests are needed to complement ultrasound scanning, the major modality for surveillance of groups at high risk of HCC. We have analysed the serum proteome of 182 patients with hepatitis C-induced liver cirrhosis (77 with HCC) by surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI). The patients were split into a training set (84 non-HCC, 60 HCC) and a 'blind' test set (21 non-HCC, 17 HCC). Neural networks developed on the training set were able to classify the blind test set with 94% sensitivity (95% CI 73-99%) and 86% specificity (95% CI 65-95%). Two of the SELDI peaks (23/23.5 kDa) were elevated by an average of 50% in the serum of HCC patients (P
Original languageEnglish
Pages (from-to)287-292
Number of pages6
JournalBritish Journal of Cancer
Volume94
DOIs
Publication statusPublished - 10 Jan 2006

Keywords

  • SELDI
  • proteome
  • serum
  • hepatocellular carcinoma

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