Changes in human hepatic metabolism in steatosis and cirrhosis

Zoe Schofield, Michelle A.C. Reed, Philip Newsome, David Adams, Ulrich Gunther, Patricia Lalor

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)
221 Downloads (Pure)

Abstract

AIM: To understand the underlying metabolic changes in human liver disease we have applied nuclear magnetic resonance (NMR) metabolomics analysis to human liver tissue.

METHODS: We have carried out pilot study using (1)H-NMR to derive metabolomic signatures from human liver from patients with steatosis, nonalcoholic steatohepatitis (NASH) or alcohol-related liver damage (ARLD) to identify species that can predict outcome and discriminate between alcohol and metabolic-induced liver injuries.

RESULTS: Changes in branched chain amino acid homeostasis, tricarboxylic acid cycle and purine biosynthesis intermediates along with betaine were associated with the development of cirrhosis in both ARLD and nonalcoholic fatty liver disease. Species such as propylene glycol and as yet unidentified moieties that allowed discrimination between NASH and ARLD samples were also detected using our approach.

CONCLUSION: Our high throughput, non-destructive technique for multiple analyte quantification in human liver specimens has potential for identification of biomarkers with prognostic and diagnostic significance.

Original languageEnglish
Pages (from-to)2685-2695
Number of pages11
JournalWorld Journal of Gastroenterology
Volume23
Issue number15
DOIs
Publication statusPublished - 21 Apr 2017

Keywords

  • Journal Article

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