cDNA cloning and expression of a cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus

Young-Mi Lee; Timothy Williams; Sang-Oun Jung; Jae-Seong Lee

doi:10.1016/j.marpolbul.2005.06.015

cDNA cloning and expression of a cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus

Young-Mi Lee, Timothy Williams, Sang-Oun Jung, Jae-Seong Lee

Biosciences

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Abstract

We previously described the genomic structure of the cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus [Kim, I.-C., Kim, Y.J., Yoon, Y.-D, Kawamura, S., Lee, Y.-S., Lee, J.-S., 2004a. Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphroditic fish Rivulus marmoratus and the Japanese medaka Oryzias latipes. Mar. Environ. Res. 58, 125–129]. To further characterize R. marmoratus CYP1A, we cloned the cDNA sequence of a CYP1A gene from this species and also expressed its recombinant protein in an E. coli system. We exposed R. marmoratus to 4-nonylphenol, and found a small induction of CYP1A mRNA in the treated animals. In this paper, we discuss the characteristics of R. marmoratus CYP1A gene as well as its potential use in a biomonitoring assay.

Original language	English
Pages (from-to)	769-775
Journal	Marine Pollution Bulletin
Volume	51
Issue number	8-12
DOIs	https://doi.org/10.1016/j.marpolbul.2005.06.015
Publication status	Published - 14 Jul 2005

Keywords

Rivulus marmoratus
CYP1A
Escherichia coli
cDNA
Expression

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10.1016/j.marpolbul.2005.06.015

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title = "cDNA cloning and expression of a cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus",

abstract = "We previously described the genomic structure of the cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus [Kim, I.-C., Kim, Y.J., Yoon, Y.-D, Kawamura, S., Lee, Y.-S., Lee, J.-S., 2004a. Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphroditic fish Rivulus marmoratus and the Japanese medaka Oryzias latipes. Mar. Environ. Res. 58, 125–129]. To further characterize R. marmoratus CYP1A, we cloned the cDNA sequence of a CYP1A gene from this species and also expressed its recombinant protein in an E. coli system. We exposed R. marmoratus to 4-nonylphenol, and found a small induction of CYP1A mRNA in the treated animals. In this paper, we discuss the characteristics of R. marmoratus CYP1A gene as well as its potential use in a biomonitoring assay.",

keywords = "Rivulus marmoratus, CYP1A, Escherichia coli, cDNA, Expression",

author = "Young-Mi Lee and Timothy Williams and Sang-Oun Jung and Jae-Seong Lee",

year = "2005",

month = jul,

day = "14",

doi = "10.1016/j.marpolbul.2005.06.015",

language = "English",

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journal = "Marine Pollution Bulletin",

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TY - JOUR

T1 - cDNA cloning and expression of a cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus

AU - Lee, Young-Mi

AU - Williams, Timothy

AU - Jung, Sang-Oun

AU - Lee, Jae-Seong

PY - 2005/7/14

Y1 - 2005/7/14

N2 - We previously described the genomic structure of the cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus [Kim, I.-C., Kim, Y.J., Yoon, Y.-D, Kawamura, S., Lee, Y.-S., Lee, J.-S., 2004a. Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphroditic fish Rivulus marmoratus and the Japanese medaka Oryzias latipes. Mar. Environ. Res. 58, 125–129]. To further characterize R. marmoratus CYP1A, we cloned the cDNA sequence of a CYP1A gene from this species and also expressed its recombinant protein in an E. coli system. We exposed R. marmoratus to 4-nonylphenol, and found a small induction of CYP1A mRNA in the treated animals. In this paper, we discuss the characteristics of R. marmoratus CYP1A gene as well as its potential use in a biomonitoring assay.

AB - We previously described the genomic structure of the cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus [Kim, I.-C., Kim, Y.J., Yoon, Y.-D, Kawamura, S., Lee, Y.-S., Lee, J.-S., 2004a. Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphroditic fish Rivulus marmoratus and the Japanese medaka Oryzias latipes. Mar. Environ. Res. 58, 125–129]. To further characterize R. marmoratus CYP1A, we cloned the cDNA sequence of a CYP1A gene from this species and also expressed its recombinant protein in an E. coli system. We exposed R. marmoratus to 4-nonylphenol, and found a small induction of CYP1A mRNA in the treated animals. In this paper, we discuss the characteristics of R. marmoratus CYP1A gene as well as its potential use in a biomonitoring assay.

KW - Rivulus marmoratus

KW - CYP1A

KW - Escherichia coli

KW - cDNA

KW - Expression

U2 - 10.1016/j.marpolbul.2005.06.015

DO - 10.1016/j.marpolbul.2005.06.015

M3 - Article

SN - 0025-326X

VL - 51

SP - 769

EP - 775

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

IS - 8-12

ER -

cDNA cloning and expression of a cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus

Abstract

Keywords

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