CdbA is a DNA-binding protein and c-di-GMP receptor important for nucleoid organization and segregation in Myxococcus xanthus

Dorota Skotnicka, Wieland Steinchen, Dobromir Szadkowski, Ian T. Cadby, Andrew L. Lovering, Gert Bange, Lotte Søgaard-andersen

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
93 Downloads (Pure)

Abstract

Cyclic di-GMP (c-di-GMP) is a second messenger that modulates multiple responses to environmental and cellular signals in bacteria. Here we identify CdbA, a DNA-binding protein of the ribbon-helix-helix family that binds c-di-GMP in Myxococcus xanthus. CdbA is essential for viability, and its depletion causes defects in chromosome organization and segregation leading to a block in cell division. The protein binds to the M. xanthus genome at multiple sites, with moderate sequence specificity; however, its depletion causes only modest changes in transcription. The interactions of CdbA with c-di-GMP and DNA appear to be mutually exclusive and residue substitutions in CdbA regions important for c-di-GMP binding abolish binding to both c-di-GMP and DNA, rendering these protein variants non-functional in vivo. We propose that CdbA acts as a nucleoid-associated protein that contributes to chromosome organization and is modulated by c-di-GMP, thus revealing a link between c-di-GMP signaling and chromosome biology.
Original languageEnglish
Article number1791
Number of pages19
JournalNature Communications
Volume11
Issue number1
DOIs
Publication statusPublished - 14 Apr 2020

Keywords

  • Bacterial Proteins/chemistry
  • Base Sequence
  • Cell Nucleus/metabolism
  • Chromosome Segregation
  • Chromosomes, Bacterial/metabolism
  • Cyclic GMP/analogs & derivatives
  • DNA, Bacterial/metabolism
  • DNA-Binding Proteins/metabolism
  • Genetic Loci
  • Models, Molecular
  • Myxococcus xanthus/metabolism
  • Protein Multimerization
  • Protein Structure, Secondary
  • Transcription, Genetic

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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