CD117(+)CD3(-)CD56(-)OX40L(high) cells express IL-22 and display an LTi phenotype in human secondary lymphoid tissues

S Kim, S Han, David Withers, Fabrina Gaspal, J Bae, S Baik, HC Shin, KS Kim, V Bekiaris, Graham Anderson, Peter Lane, MY Kim

Research output: Contribution to journalArticle

34 Citations (Scopus)


Here, we identify cells within human adult secondary lymphoid tissues that are comparable in phenotype and location to the lymphoid tissue inducer (LTi) cells that persist in the adult mouse. Identified as CD117(+)CD3(-)CD56(-) cells, like murine LTi cells, they lack expression of many common lineage markers and express CD127, OX40L and TRANCE. These cells were detected at the interface between the B- and T- zones, as well as at the subcapsular sinus in LNs, the location where LTi cells reside in murine spleen and LNs. Furthermore, like murine LTi cells, these cells expressed high levels of IL-22 and upregulated IL-22 expression upon IL-23 stimulation. Importantly, these cells were not an NK cell subset since they showed no expression of IFN-gamma and perforin. Interestingly, a subset of the CD117(+)CD3(-)CD56(-)OX40L(+) population expressed NKp46, again similar to recent findings in mice. Finally, these cells supported memory CD4(+) T-cell survival in an OX40L-dependent manner. Combined, these data indicate that the CD117(+)CD3(-)CD56(-)OX40L(+) cells in human secondary lymphoid tissues are comparable in phenotype, location and function to the LTi cells that persist within adult murine secondary lymphoid tissues.
Original languageEnglish
Pages (from-to)1563-1572
Number of pages10
JournalEuropean Journal of Immunology
Issue number6
Publication statusPublished - 1 Jun 2011


  • CD117(+) CD3 CD56 OX40L(+)
  • T-cell survival
  • Tonsil


Dive into the research topics of 'CD117(+)CD3(-)CD56(-)OX40L(high) cells express IL-22 and display an LTi phenotype in human secondary lymphoid tissues'. Together they form a unique fingerprint.

Cite this