CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes

Emma C. Mackley; Stephanie Houston; Clare L. Marriott; Emily E. Halford; Beth Lucas; Vuk Cerovic; Kara J. Filbey; Rick M. Maizels; Matthew R. Hepworth; Gregory F. Sonnenberg; Simon Milling; David R. Withers

doi:10.1038/ncomms6862

CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes

Emma C. Mackley, Stephanie Houston, Clare L. Marriott, Emily E. Halford, Beth Lucas, Vuk Cerovic, Kara J. Filbey, Rick M. Maizels, Matthew R. Hepworth, Gregory F. Sonnenberg, Simon Milling, David R. Withers

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

108 Citations (Scopus)

277 Downloads (Pure)

Abstract

Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.

Original language	English
Article number	5862
Journal	Nature Communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms6862
Publication status	Published - 9 Jan 2015

Keywords

Biological sciences
Immunology

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Dissecting the Role of Lymphoid Tissue Inducer Cells in Lymph Node Responses
Withers, D.
THE WELLCOME TRUST
1/04/11 → 31/03/16
Project: Research
MRC Centre For Immune Regulation (Linked to DCDF.RRAK10540) (Linked to 14810 & 14835)
Jenkinson, E.
Medical Research Council
3/08/09 → 30/09/17
Project: Research Councils

Cite this

Mackley, E. C., Houston, S., Marriott, C. L., Halford, E. E., Lucas, B., Cerovic, V., Filbey, K. J., Maizels, R. M., Hepworth, M. R., Sonnenberg, G. F., Milling, S., & Withers, D. R. (2015). CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes. Nature Communications, 6, Article 5862. https://doi.org/10.1038/ncomms6862

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title = "CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes",

abstract = "Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.",

keywords = "Biological sciences, Immunology",

author = "Mackley, {Emma C.} and Stephanie Houston and Marriott, {Clare L.} and Halford, {Emily E.} and Beth Lucas and Vuk Cerovic and Filbey, {Kara J.} and Maizels, {Rick M.} and Hepworth, {Matthew R.} and Sonnenberg, {Gregory F.} and Simon Milling and Withers, {David R.}",

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AU - Mackley, Emma C.

AU - Houston, Stephanie

AU - Marriott, Clare L.

AU - Halford, Emily E.

AU - Lucas, Beth

AU - Cerovic, Vuk

AU - Filbey, Kara J.

AU - Maizels, Rick M.

AU - Hepworth, Matthew R.

AU - Sonnenberg, Gregory F.

AU - Milling, Simon

AU - Withers, David R.

PY - 2015/1/9

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N2 - Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.

AB - Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.

KW - Biological sciences

KW - Immunology

UR - https://www.nature.com/articles/ncomms11186

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DO - 10.1038/ncomms6862

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VL - 6

JO - Nature Communications

JF - Nature Communications

M1 - 5862

ER -

CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes

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Projects

Dissecting the Role of Lymphoid Tissue Inducer Cells in Lymph Node Responses

MRC Centre For Immune Regulation (Linked to DCDF.RRAK10540) (Linked to 14810 & 14835)

Cite this