Carbamylated LL-37 as a modulator of the immune response

Catalin Koro, Annelie Hellvard, Nicolas Delaleu, Veronika Binder, Carsten Scavenius, Brith Bergum, Izabela Główczyk, Helen Roberts, Iain Chapple, Melissa Grant, Maria Rapala-Kozik, Kinga Klaga, Jan Enghild, Piotr Mydel

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)
199 Downloads (Pure)


Carbamylation of lysine residues and protein N-termini is a ubiquitous, non-enzymatic post-translational modification. Carbamylation at sites of inflammation is due to cyanate formation during the neutrophil oxidative burst and may target lysine residues within the antimicrobial peptide LL-37. The bactericidal and immunomodulatory properties of LL-37 depend on its secondary structure and cationic nature, which are conferred by arginine and lysine residues. Therefore, carbamylation may affect the biological functions of LL-37. The present study examined the kinetics and pattern of LL-37 carbamylation to investigate how this modification affects the bactericidal, cytotoxic, and immunomodulatory function of the peptide. The results indicated that LL-37 undergoes rapid modification in the presence of physiological concentrations of cyanate, yielding a spectrum of diverse carbamylated peptides. Mass spectrometry analyses revealed that the N-terminal amino group of Leu-1 was highly reactive and was modified almost instantly by cyanate to generate the predominant form of the modified peptide, named LL37C1. This was followed by the sequential carbamylation of Lys-8, Lys-12, and Lys-15, to yield LL37C8, and LL37C12,15, respectively. Carbamylation had profound and diverse effects on the structure and biological properties of LL-37. In some cases, anti-inflammatory LL-37 was rapidly converted to pro-inflammatory LL-37.
Original languageEnglish
JournalInnate immunity
Early online date15 Feb 2016
Publication statusPublished - 22 Mar 2016


Dive into the research topics of 'Carbamylated LL-37 as a modulator of the immune response'. Together they form a unique fingerprint.

Cite this