cAMP signaling microdomains and their observation by optical methods

Davide Calebiro, Isabella Maiellaro

Research output: Contribution to journalReview articlepeer-review

32 Citations (Scopus)
154 Downloads (Pure)


The second messenger cyclic AMP (cAMP) is a major intracellular mediator of many hormones and neurotransmitters and regulates a myriad of cell functions, including synaptic plasticity in neurons. Whereas cAMP can freely diffuse in the cytosol, a growing body of evidence suggests the formation of cAMP gradients and microdomains near the sites of cAMP production, where cAMP signals remain apparently confined. The mechanisms responsible for the formation of such microdomains are subject of intensive investigation. The development of optical methods based on fluorescence resonance energy transfer (FRET), which allow a direct observation of cAMP signaling with high temporal and spatial resolution, is playing a fundamental role in elucidating the nature of such microdomains. Here, we will review the optical methods used for monitoring cAMP and protein kinase A (PKA) signaling in living cells, providing some examples of their application in neurons, and will discuss the major hypotheses on the formation of cAMP/PKA microdomains.

Original languageEnglish
Article number350
Number of pages9
JournalFrontiers in Cellular Neuroscience
Issue numberOctober
Publication statusPublished - 28 Oct 2014


  • Cyclic AMP
  • Fluorescence resonance energy transfer
  • G protein-coupled receptor
  • Neurons
  • Signaling microdomain

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience


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