TY - JOUR
T1 - Calcium signalling in pollen of Papaver rhoeas undergoing the self-incompatibility (SI) response
AU - Straatman, Kornelis
AU - Dove, Stephen
AU - Holdaway Clarke, T
AU - Helper, PK
AU - Kunkel, JG
AU - Franklin Tong, Vernonica
PY - 2001/9/1
Y1 - 2001/9/1
N2 - Self-incompatibility (SI) is a genetically controlled system used by many flowering plants to prevent self-pollination. We established, using calcium imaging, that the SI response in Papaver rhoeas L. (poppy) pollen involves a Ca2+-mediated intracellular signalling pathway. Here we review what is known about the signalling components and cascades implicated in the SI response in poppy pollen. We present some studies using calcium green (CG-1) that show SI-induced alterations in CG-1 fluorescence and localization. We have begun to examine potential sources of Ca2+ involved in the responses induced by SI. This work presents preliminary data showing that influx of extracellular Ca2+ at the "shank" of the pollen tube is possible. This is the first evidence suggesting that influx at this localization may play a role in the SI response. We also describe preliminary studies that begin to investigate whether the phosphoinositide signalling pathway is implicated in the SI response.
AB - Self-incompatibility (SI) is a genetically controlled system used by many flowering plants to prevent self-pollination. We established, using calcium imaging, that the SI response in Papaver rhoeas L. (poppy) pollen involves a Ca2+-mediated intracellular signalling pathway. Here we review what is known about the signalling components and cascades implicated in the SI response in poppy pollen. We present some studies using calcium green (CG-1) that show SI-induced alterations in CG-1 fluorescence and localization. We have begun to examine potential sources of Ca2+ involved in the responses induced by SI. This work presents preliminary data showing that influx of extracellular Ca2+ at the "shank" of the pollen tube is possible. This is the first evidence suggesting that influx at this localization may play a role in the SI response. We also describe preliminary studies that begin to investigate whether the phosphoinositide signalling pathway is implicated in the SI response.
KW - calcium influx
KW - pollen
KW - calcium signalling
KW - self-incompatibility
KW - inositide signalling
UR - http://www.scopus.com/inward/record.url?scp=0034815698&partnerID=8YFLogxK
U2 - 10.1007/s004970100092
DO - 10.1007/s004970100092
M3 - Article
SN - 1432-2145
SN - 1432-2145
SN - 1432-2145
VL - 14
SP - 105
EP - 110
JO - Sexual Plant Reproduction
JF - Sexual Plant Reproduction
ER -