Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections

Arianna Castoldi, Christian Herr, Julia Niederstraßer, Hagar Ibrahim Labouta, Ana Melero, Sarah Gordon, Nicole Schneider-Daum, Robert Bals, Claus Michael Lehr

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The influence of vitamin D3 and its metabolites calcifediol (25(OH)D) and calcitriol on immune regulation and inflammation is well described, and raises the question of potential benefit against bacterial infections. In the current study, 25(OH)D was encapsulated in liposomes to enable aerosolisation, and tested for the ability to prevent pulmonary infection by Pseudomonas aeruginosa. Prepared 25(OH)D-loaded liposomes were nanosized and monodisperse, with a negative surface charge and a 25(OH)D entrapment efficiency of approximately 23%. Jet nebulisation of liposomes was seen to yield an aerosol suitable for tracheo-bronchial deposition. Interestingly, 25(OH)D in either liposomes or ethanolic solution had no effect on the release of the proinflammatory cytokine KC from Pseudomonas-infected murine epithelial cells (LA-4); treatment of infected, human bronchial 16-HBE cells with 25(OH)D liposomes however resulted in a significant reduction in bacterial survival. Together with the importance of selecting an application-appropriate in vitro model, the current study illustrates the feasibility and practicality of employing liposomes as a means to achieve 25(OH)D lung deposition. 25(OH)D-loaded liposomes further demonstrated promising effects regarding prevention of Pseudomonas infection in human bronchial epithelial cells.

Original languageEnglish
Pages (from-to)62-67
Number of pages6
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume118
Early online date22 Nov 2016
DOIs
Publication statusPublished - Sep 2017

Bibliographical note

Publisher Copyright:
© 2016 Elsevier B.V.

Keywords

  • Calcifediol
  • Cystic fibrosis
  • Liposome aerosol
  • Poorly soluble drugs
  • Pseudomonas aeruginosa
  • Pulmonary drug delivery

ASJC Scopus subject areas

  • Biotechnology
  • Pharmaceutical Science

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