BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation

Alasdair Gawain Kay, Tina Patricia Dale, Khondoker Mehedi Akram, Param Mohan, Karen Hampson, Nicola Maffulli, Monica A Spiteri, Alicia Jennifer El Haj, Nicholas Robert Forsyth

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


AIM: Human mesenchymal stem cells (hMSC) are multipotent progenitor cells. We propose the optimization of hMSC isolation and recovery using the application of a controlled hypoxic environment.

MATERIALS & METHODS: We evaluated oxygen, glucose and serum in the recovery of hMSC from bone marrow (BMhMSC). Colony forming units-fibroblastic, cell numbers, tri-lineage differentiation, immunofluorescence and microarray were used to confirm and characterize BMhMSC.

RESULTS: In an optimized (2% O(2), 4.5 g/l glucose and 5% serum) environment both colony forming units-fibroblastic (p = 0.01) and cell numbers (p = 0.0001) were enhanced over standard conditions. Transcriptional analysis identified differential expression of bone morphogenetic protein 2 (BMP2) and, putatively, chemokine (C-X-C motif) receptor 2 (CXCR2) signaling pathways.

CONCLUSION: We have detailed a potential milestone in the process of refinement of the BMhMSC isolation process.

Original languageEnglish
Pages (from-to)109-125
Number of pages17
JournalRegenerative Medicine
Issue number2
Publication statusPublished - Mar 2015


  • Bone Marrow/pathology
  • Bone Marrow Cells/cytology
  • Bone Morphogenetic Protein 2/metabolism
  • Cell Culture Techniques
  • Cell Differentiation
  • Cell Hypoxia
  • Cell Proliferation
  • Cells, Cultured
  • Chemokines/metabolism
  • Colony-Forming Units Assay
  • Computational Biology/methods
  • Glucose/chemistry
  • Humans
  • Immunophenotyping
  • Mesenchymal Stromal Cells/cytology
  • Microscopy, Fluorescence
  • Oligonucleotide Array Sequence Analysis
  • Osteoblasts/cytology
  • Oxygen/chemistry
  • Signal Transduction
  • Transcriptome
  • Up-Regulation
  • BMP2
  • CFU-F
  • hypoxia
  • mesenchymal stem cells
  • physiological normoxia


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