Biotinylated boronic acid fluorophore conjugates: Quencher elimination strategy for imaging and saccharide detection

F D'Hooge; SA Elfeky; SE Flower; SI Pascu; ATA Jenkins; JMH van den Elsen; TD James; John S. Fossey

doi:10.1039/c2ra00542e

Biotinylated boronic acid fluorophore conjugates: Quencher elimination strategy for imaging and saccharide detection

F D'Hooge, SA Elfeky, SE Flower, SI Pascu, ATA Jenkins, JMH van den Elsen, TD James, John S. Fossey

Chemistry

Research output: Contribution to journal › Article

18 Citations (Scopus)

Abstract

A biotinylated boronic acid fluorophore conjugate bound to a diol-quencher via a boronic ester linkage demonstrated a fluorescence increase upon exposure to model saccharides. The sensing motif was attached to SuperAvidin microspheres where the sensing regime was imaged by fluorescence microscopy.

Original language	English
Pages (from-to)	3274-3280
Number of pages	7
Journal	RSC Advances
Volume	2
Issue number	8
DOIs	https://doi.org/10.1039/c2ra00542e
Publication status	Published - 1 Jan 2012

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title = "Biotinylated boronic acid fluorophore conjugates: Quencher elimination strategy for imaging and saccharide detection",

abstract = "A biotinylated boronic acid fluorophore conjugate bound to a diol-quencher via a boronic ester linkage demonstrated a fluorescence increase upon exposure to model saccharides. The sensing motif was attached to SuperAvidin microspheres where the sensing regime was imaged by fluorescence microscopy.",

author = "F D'Hooge and SA Elfeky and SE Flower and SI Pascu and ATA Jenkins and {van den Elsen}, JMH and TD James and Fossey, {John S.}",

year = "2012",

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AU - D'Hooge, F

AU - Elfeky, SA

AU - Flower, SE

AU - Pascu, SI

AU - Jenkins, ATA

AU - van den Elsen, JMH

AU - James, TD

AU - Fossey, John S.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - A biotinylated boronic acid fluorophore conjugate bound to a diol-quencher via a boronic ester linkage demonstrated a fluorescence increase upon exposure to model saccharides. The sensing motif was attached to SuperAvidin microspheres where the sensing regime was imaged by fluorescence microscopy.

AB - A biotinylated boronic acid fluorophore conjugate bound to a diol-quencher via a boronic ester linkage demonstrated a fluorescence increase upon exposure to model saccharides. The sensing motif was attached to SuperAvidin microspheres where the sensing regime was imaged by fluorescence microscopy.

U2 - 10.1039/c2ra00542e

DO - 10.1039/c2ra00542e

M3 - Article

VL - 2

SP - 3274

EP - 3280

JO - RSC Advances

JF - RSC Advances

IS - 8

ER -

Biotinylated boronic acid fluorophore conjugates: Quencher elimination strategy for imaging and saccharide detection

Abstract

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