Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp. SANK 73390

Annabel C. Murphy, Shu Shan Gao, Li Chen Han, Simon Carobene, Daisuke Fukuda, Zhongshu Song, Joanne Hothersall, Russell J. Cox, John Crosby, Matthew P. Crump, Christine L. Willis*, Thomas J. Simpson, Christopher Thomas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


The biosynthesis of the mixed PKS-FAS-NRPS hybrid antibiotic thiomarinol A was investigated using feeding studies to both wild type and mutant strains of the marine bacterium Pseudoalteromonas. Particularly interesting features of the pathway include assembly of the 8-hydroxyoctanoic acid side-chain via chain extension of a C4-precursor (4-hydroxybutyrate), and construction of the pyrrothine unit from cysteine via (HolA-D, F-H) prior to intact incorporation into thiomarinol (catalysed by TmlU). A series of thiomarinol-related and other minor metabolites have been isolated from wild-type and mutant strains. The results of these investigations are rationalised in terms of the overall biosynthetic pathway.

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalChemical Science
Issue number1
Publication statusPublished - Jan 2014

ASJC Scopus subject areas

  • General Chemistry


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