Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

Shu Shan Gao, Joanne Hothersall, Ji'En Wu, Annabel C. Murphy, Zhongshu Song, Elton R. Stephens, Christopher M. Thomas, Matthew P. Crump, Russell J. Cox, Thomas J. Simpson*, Christine L. Willis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.

Original languageEnglish
Pages (from-to)5501-5507
Number of pages7
JournalJournal of the American Chemical Society
Issue number14
Early online date27 Mar 2014
Publication statusPublished - 9 Apr 2014

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry
  • Medicine(all)


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