Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

Shu Shan Gao; Joanne Hothersall; Ji'En Wu; Annabel C. Murphy; Zhongshu Song; Elton R. Stephens; Christopher M. Thomas; Matthew P. Crump; Russell J. Cox; Thomas J. Simpson; Christine L. Willis

doi:10.1021/ja501731p

Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

Shu Shan Gao, Joanne Hothersall, Ji'En Wu, Annabel C. Murphy, Zhongshu Song, Elton R. Stephens, Christopher M. Thomas, Matthew P. Crump, Russell J. Cox, Thomas J. Simpson^*, Christine L. Willis

^*Corresponding author for this work

Biosciences

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20 Citations (Scopus)

Abstract

Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.

Original language	English
Pages (from-to)	5501-5507
Number of pages	7
Journal	Journal of the American Chemical Society
Volume	136
Issue number	14
Early online date	27 Mar 2014
DOIs	https://doi.org/10.1021/ja501731p
Publication status	Published - 9 Apr 2014

ASJC Scopus subject areas

Chemistry(all)
Catalysis
Biochemistry
Colloid and Surface Chemistry
Medicine(all)

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10.1021/ja501731pLicence: None: All rights reserved

Novel hybrid anti-MRSA antibiotics from manipulation of the mupirocin and thiomarinol biosynthetic pathways
Thomas, C. & Winn, P.
Biotechnology & Biological Sciences Research Council
1/10/11 → 31/12/14
Project: Research Councils
Biosynthesis of polyketide antibiotic mupirocin by Pseudomonas fluorescens
Thomas, C.
Biotechnology & Biological Sciences Research Council
1/06/07 → 30/01/11
Project: Research Councils
Biosynthesis of the Polyketide Antibiotic Mupirocin by Pseudomonas Fluorescens
Thomas, C. & Hothersall, J.
Biotechnology & Biological Sciences Research Council
14/05/04 → 13/05/07
Project: Research Councils

Cite this

Gao, S. S., Hothersall, J., Wu, JE., Murphy, A. C., Song, Z., Stephens, E. R., Thomas, C. M., Crump, M. P., Cox, R. J., Simpson, T. J., & Willis, C. L. (2014). Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways. Journal of the American Chemical Society, 136(14), 5501-5507. Advance online publication. https://doi.org/10.1021/ja501731p

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abstract = "Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.",

author = "Gao, {Shu Shan} and Joanne Hothersall and Ji'En Wu and Murphy, {Annabel C.} and Zhongshu Song and Stephens, {Elton R.} and Thomas, {Christopher M.} and Crump, {Matthew P.} and Cox, {Russell J.} and Simpson, {Thomas J.} and Willis, {Christine L.}",

year = "2014",

month = apr,

day = "9",

doi = "10.1021/ja501731p",

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journal = "Journal of the American Chemical Society",

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AU - Gao, Shu Shan

AU - Hothersall, Joanne

AU - Wu, Ji'En

AU - Murphy, Annabel C.

AU - Song, Zhongshu

AU - Stephens, Elton R.

AU - Thomas, Christopher M.

AU - Crump, Matthew P.

AU - Cox, Russell J.

AU - Simpson, Thomas J.

AU - Willis, Christine L.

PY - 2014/4/9

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AB - Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.

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Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways

Abstract

ASJC Scopus subject areas

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Projects

Novel hybrid anti-MRSA antibiotics from manipulation of the mupirocin and thiomarinol biosynthetic pathways

Biosynthesis of polyketide antibiotic mupirocin by Pseudomonas fluorescens

Biosynthesis of the Polyketide Antibiotic Mupirocin by Pseudomonas Fluorescens

Cite this