Biomodulatory effects of laser irradiation on dental pulp cells in vitro

Michael R. Milward*, Mohammed A. Hadis, Paul R. Cooper, Patricia Gorecki, James D. Carroll, William M. Palin

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)


Low level laser/light therapy (LLLT) or photobiomodulation is a biophysical approach that can be used to reduce pain, inflammation and modulate tissue healing and repair. However, its application has yet to be fully realized for dental disease treatment. The aim of this study was to assess the modulation of dental pulp cell (DPC) responses using two LLLT lasers with wavelengths of 660nm and 810nm. Human DPCs were isolated and cultured in phenol-red-free α-MEM/10%-FCS at 37°C in 5% CO2. Central wells of transparent-based black walled 96-microplates were seeded with DPCs (passages 2-4; 150μ1/4L; 25,000 cell/ml). At 24h post-seeding, cultures were irradiated using a Thor Photomedicine LLLT device (THOR Photomedicine, UK) at 660nm (3, 6 or 13s to give 2, 5 and 10J/cm2) or 810nm (for 1, 2 or 5s to deliver 5, 10 and 20J/cm2). Metabolic activity was assessed via a modified MTT assay 24h post-irradiation. Statistical differences were identified using analysis of variance and post-hoc Tukey tests (P=0.05) and compared with nonirradiated controls. Significantly higher MTT activity was obtained for both lasers (P<0.05) using the high and intermediate radiant exposure (5-20J/cm2). The MTT response significantly decreased (P<0.05) at lower radiant exposures with no statistical significance from control (P>0.05). Consequently, enhanced irradiation parameters was apparent for both lasers. These parameters should be further optimised to identify the most effective for therapeutic application.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSociety of Photo-Optical Instrumentation Engineers
ISBN (Print)9781628413991
Publication statusPublished - 2015
EventMechanisms for Low-Light Therapy X - San Francisco, United States
Duration: 7 Feb 20158 Feb 2015


ConferenceMechanisms for Low-Light Therapy X
Country/TerritoryUnited States
CitySan Francisco


  • beam profile
  • dose
  • LLLT
  • MTT
  • photobiomodulation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


Dive into the research topics of 'Biomodulatory effects of laser irradiation on dental pulp cells in vitro'. Together they form a unique fingerprint.

Cite this