Bioluminescence tomography improves quantitative accuracy for pre-clinical imaging

J.A. Guggenheim; H.R.A. Basevi; H. Dehghani; I.B. Styles; J. Frampton

doi:10.1117/12.2033317

Bioluminescence tomography improves quantitative accuracy for pre-clinical imaging

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Abstract

A study is presented that demonstrates that bioluminescence tomography can reconstruct accurate 3D images of internal light sources placed at a range of depths within a physical phantom and that it provides more reliable quantitative data than standard bioluminescence imaging. Specifically, it is shown that when imaging sources at depths ranging from 5 to 15mm, estimates of total source strength are stable to within ±11% using tomography whilst values deduced by traditional methods vary 10-fold. Additionally, the tomographic approach correctly localises sources to within 1.5mm error in all cases considered.

Original language	English
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8799
DOIs	https://doi.org/10.1117/12.2033317
Publication status	Published - 25 Jun 2013

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guggenheim2013bioluminescence
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Bioluminescence tomography improves quantitative accuracy for pre-clinical imaging

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