@inbook{baf23b25f7f24f1b80b4ad8409092a68,
title = "Bioluminescence Resonance Energy Transfer (BRET) Assay to Measure Gq Recruitment to the Ghrelin Receptor",
abstract = "This protocol chapter outlines a guide for investigating ghrelin receptor activation of Gq signaling using Bioluminescence Resonance Energy Transfer (BRET). Focusing on the eBRET2 and NanoBRET{\texttrademark} variants, the chapter covers steps from cell culture to transfection, ligand stimulation, and BRET measurements, offering a robust protocol to examine the temporal aspects of Gq signaling in live cells. This methodology facilitates a nuanced understanding of Gq-mediated cellular responses, but also provides researchers with a valuable tool for dissecting other related signaling pathways.",
author = "Aqfan Jamaluddin",
year = "2024",
month = oct,
day = "13",
doi = "10.1007/978-1-0716-4164-4_5",
language = "English",
isbn = "9781071641668",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "57--69",
editor = "Gorvin, {Caroline M. }",
booktitle = "Calcium Signaling",
edition = "1",
}