Biological in situ characterization of polymeric microbubble contrast agents

Sha Wan, Gabriella Egri, Letizia Oddo, Barbara Cerroni, Lars Dähne, Gaio Paradossi, Anna Salvati, Iseult Lynch, Kenneth A. Dawson, Marco P. Monopoli

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10 Citations (Scopus)
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Abstract

Polymeric microbubbles (MBs) are gas filled particles composed of a thin stabilized polymer shell that have been recently developed as valid contrast agents for the combined use of ultrasonography (US), magnetic resonance imaging (MRI) and single photon emission computer tomography (SPECT) imaging. Due to their buoyancy, the commonly available approaches to study their behaviour in complex media are not easily applicable and their use in modern medicine requires such behaviour to be fully elucidated. Here we have used for the first time flow cytometry as a new high throughput approach that allows characterisation of the MB dispersion, prior to and after exposure in different biological media and we have additionally developed a method that allows characterisation of the strongly bound proteins adsorbed on the MBs, to fully predict their biological behaviour in biological milieu.
Original languageEnglish
JournalThe International Journal of Biochemistry & Cell Biology
Early online date16 Mar 2016
DOIs
Publication statusE-pub ahead of print - 16 Mar 2016

Keywords

  • Microbubbles
  • Protein corona
  • Flow cytometry
  • Opsonin proteins
  • Dysopsonin proteins
  • Bionano interactions

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