Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR.

S Samarasinghe; MS El-Robh; David Grainger; W Zhang; P Soultanas; Stephen Busby

doi:10.1093/nar/gkn119

Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR.

S Samarasinghe, MS El-Robh, David Grainger, W Zhang, P Soultanas, Stephen Busby

Biosciences

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Abstract

The Escherichia coli MelR protein is a transcription activator that autoregulates its own promoter by repressing transcription initiation. Optimal repression requires MelR binding to a site that overlaps the melR transcription start point and to upstream sites. In this work, we have investigated the different determinants needed for optimal repression and their spatial requirements. We show that repression requires a complex involving four DNA-bound MelR molecules, and that the global CRP regulator plays little or no role.

Original language	English
Pages (from-to)	2667-76
Number of pages	10
Journal	Nucleic Acids Research
Volume	36
Issue number	8
DOIs	https://doi.org/10.1093/nar/gkn119
Publication status	Published - 1 May 2008

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busby_200801.pdf

http://nar.oxfordjournals.org/cgi/content/full/36/8/2667

Molecular Mechanism of Triggering of a Bacterial Transcription Activator
Busby, S.
Biotechnology & Biological Sciences Research Council
1/12/04 → 14/09/08
Project: Research Councils

Cite this

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abstract = "The Escherichia coli MelR protein is a transcription activator that autoregulates its own promoter by repressing transcription initiation. Optimal repression requires MelR binding to a site that overlaps the melR transcription start point and to upstream sites. In this work, we have investigated the different determinants needed for optimal repression and their spatial requirements. We show that repression requires a complex involving four DNA-bound MelR molecules, and that the global CRP regulator plays little or no role.",

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Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR.

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Molecular Mechanism of Triggering of a Bacterial Transcription Activator

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