Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR.

S Samarasinghe, MS El-Robh, David Grainger, W Zhang, P Soultanas, Stephen Busby

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
94 Downloads (Pure)

Abstract

The Escherichia coli MelR protein is a transcription activator that autoregulates its own promoter by repressing transcription initiation. Optimal repression requires MelR binding to a site that overlaps the melR transcription start point and to upstream sites. In this work, we have investigated the different determinants needed for optimal repression and their spatial requirements. We show that repression requires a complex involving four DNA-bound MelR molecules, and that the global CRP regulator plays little or no role.
Original languageEnglish
Pages (from-to)2667-76
Number of pages10
JournalNucleic Acids Research
Volume36
Issue number8
DOIs
Publication statusPublished - 1 May 2008

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