Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.
|Title of host publication||Methods in Molecular Biology: DNA - Protein interactions|
|Editors||B LeBlanc, T Moss|
|Number of pages||19|
|Publication status||Published - 1 Jan 2009|
|Name||Methods in Molecular Biology™, Methods and Protocols|