Assays for transcription factor activity

Douglas Browning, N Savery, A Kolb, Stephen Busby

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.
Original languageEnglish
Title of host publicationMethods in Molecular Biology: DNA - Protein interactions
EditorsB LeBlanc, T Moss
PublisherHumana Press
Pages368-87
Number of pages19
Edition3rd
ISBN (Electronic)9781603270151
ISBN (Print)9781603270144
DOIs
Publication statusPublished - 1 Jan 2009

Publication series

NameMethods in Molecular Biology™, Methods and Protocols
Volume543

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