Assays for transcription factor activity

Douglas Browning; N Savery; A Kolb; Stephen Busby

doi:10.1007/978-1-60327-015-1

Assays for transcription factor activity

Douglas Browning, N Savery, A Kolb, Stephen Busby

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.

Original language	English
Title of host publication	Methods in Molecular Biology: DNA - Protein interactions
Editors	B LeBlanc, T Moss
Publisher	Humana Press
Pages	368-87
Number of pages	19
Edition	3rd
ISBN (Electronic)	9781603270151
ISBN (Print)	9781603270144
DOIs	https://doi.org/10.1007/978-1-60327-015-1
Publication status	Published - 1 Jan 2009

Publication series

Name	Methods in Molecular Biology™, Methods and Protocols
Volume	543

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10.1007/978-1-60327-015-1

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abstract = "Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.",

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AU - Kolb, A

AU - Busby, Stephen

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AB - Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.

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DO - 10.1007/978-1-60327-015-1

M3 - Chapter

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T3 - Methods in Molecular Biology™, Methods and Protocols

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BT - Methods in Molecular Biology: DNA - Protein interactions

A2 - LeBlanc, B

A2 - Moss, T

PB - Humana Press

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Assays for transcription factor activity

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